High performance liquid chromatography

High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and isopropanol; 5%, 20%, and 60%. To start, one must pour 10 ml of the Kool-Aid into a syringe that was connected to a C-18 absorbent. The plunger that came with the syringe will then be pushed through into a labeled test tube designated for the substance. Once…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

High Performance Liquid Chromatography Lab Abstract High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water,…

1.5 ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) Since from thirty years, High performance liquid Chromatography (HPLC) is well accepted technique used in laboratories worldwide. It is one of the analytical techniques used to identify, separate and quantify each analyte in a mixture. One of the primary drivers for the development of this technique has been the progression of the packing materials (particle size, packing technology, chemistry) used to impact the separation. The fundamental…

High performance liquid chromatography (HPLC) is a separation technique utilizing differences in distribution of compounds in two phases; called the stationary phase and the mobile phase. The stationary phase designates a thin layer created on the surface of fine particles and the mobile phase designates the liquid flowing over the particles. Under a certain dynamic condition, each component in a sample has a different distribution equilibrium depending upon the solubility in the phases and or…

Introduction: Acetaminophen is a common pain reliever and fever reducer used to treat conditions such as head, muscle or tooth ache, as well as colds, fevers, and even arthritis. It is available in a tablet form to be ingested. These tablets are manufactured by a large variety of pharmaceutical manufactures including a great deal of generic brand companies. These tablets all have the same active ingredient, acetaminophen, yet each hold different inactive ingredients that can impact the overall…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

rate was much higher than that in seawater. As seen in Fig.5, the polished wear scars were obtained on the wear tracks. While the scratches appeared on the wear scar of mating balls in seawater, especially, white scratches presented on the age of wear scar in Fig.5j. That result was attributed to the calcium magnesium salt precipitation (Mg(OH)2, CaCO3), which played the role of abrasive particles on the friction interface and resulted in scratches on the wear scar of balls. On the other hand,…

“DEVELOPMENT AND VALIDATION OF STABILITY INDICATING METHOD FOR SIMULTANEOUS ESTIMATION OF CEFTRIAXONE AND TAZOBACTAM INJECTION USING RP-UPLC METHOD” 2.1 OBJECTIVE To develop and Validate Stability Indicating Method for simultaneous estimation of Ceftriaxone and Tazobactam in their combined dosage form by RP-UPLC method in various stress condition like, alkaline, acidic, oxidative, thermal and photo degradation. 2.4 METHODOLOGY 2.4.1 Chromatographic Condition Column Separation was achieved by…

The chromatography was performed on an ACQUITY TM UPLC system (Waters Corp., Milford, MA, USA). The UPLC system included quaternary solvent manager, a binary pump, degasser, autosampler with an injection loop of 10 μL and a column heater-cooler. The separation was performed on Acquity UPLC BEH TM C18 column (50 × 2.1 mm, i.d., 1.7 μm, Waters, USA) maintained at 40 °C. a mobile phase of acetonitrile containing 0.1% formic acid and aqueous 5 mM ammonium acetate buffer containing 0.1% formic acid…