High Performance Liquid Chromatography Essay
These multiple paths arise due to the difference in column packing and small variations in particle size of the packing material. As different paths are of different lengths, this causes broadening of the solute band.
B-term: Longitudinal diffusion: Longitudinal diffusion is the movement of an analyte molecule outside from the center to the edges of its band, as the analyte concentration is lower at the edges than at the center of the band. Analyte diffuses from the center to the edges. Thus band broadening occurs. If the mobile phase velocity is high, then the analyte spends less time on the column, this limit outward distribution, keeping the band