Purpose
The purpose of the experiment Thin Layer Chromatography was to analyze compounds, Aspirin, a mixture of Tylenol with ethanol, acetaminophen, a mixture of Excedrin with ethanol, Aspirin, and acetaminophen, Anthracene, Benzyl, and Triphenylmethanol by separating them with the help of silica plates.
Procedure
Three silica plates were taken and on the plastic side, they were all marked 1 cm above its end using a pencil. With the help of capillary tube, the first and the second plates were spotted with Aspirin on the left and Acetaminophen on the right. The middle of the first plate was then spotted with the mixture of Tylenol and ethanol but the middle of the second plate was spotted with the mixture of Excedrin and ethanol.
On the third silica plate, the mixture of Tylenol and ethanol was spotted on the left, caffeine was spotted in the middle and the mixture of Excedrin and ethanol was spotted on the right.
Three chambers containing filter paper were taken and they were all filled with a pippet full of Ethyl Acetate. With help of a tweezers the silica plates were each placed in the chambers and the lid was closed.
When the absorbed Ethyl …show more content…
Further, it’s Rf value was calculated. When the first silica plate was observed under the UV light, it was observed that it had five spots, which concludes that it had five compounds, the third plate was observed to have four compounds, and the third has five compounds. It was observed that the spots that traveled less distance were less polar the spots that traveled more distance as their Rf value was lower than that of polar compounds. The spots were observed to have moved from its spots as they all did not travel straight some were going to the
The purpose of the experiment Thin Layer Chromatography was to analyze compounds, Aspirin, a mixture of Tylenol with ethanol, acetaminophen, a mixture of Excedrin with ethanol, Aspirin, and acetaminophen, Anthracene, Benzyl, and Triphenylmethanol by separating them with the help of silica plates.
Procedure
Three silica plates were taken and on the plastic side, they were all marked 1 cm above its end using a pencil. With the help of capillary tube, the first and the second plates were spotted with Aspirin on the left and Acetaminophen on the right. The middle of the first plate was then spotted with the mixture of Tylenol and ethanol but the middle of the second plate was spotted with the mixture of Excedrin and ethanol.
On the third silica plate, the mixture of Tylenol and ethanol was spotted on the left, caffeine was spotted in the middle and the mixture of Excedrin and ethanol was spotted on the right.
Three chambers containing filter paper were taken and they were all filled with a pippet full of Ethyl Acetate. With help of a tweezers the silica plates were each placed in the chambers and the lid was closed.
When the absorbed Ethyl …show more content…
Further, it’s Rf value was calculated. When the first silica plate was observed under the UV light, it was observed that it had five spots, which concludes that it had five compounds, the third plate was observed to have four compounds, and the third has five compounds. It was observed that the spots that traveled less distance were less polar the spots that traveled more distance as their Rf value was lower than that of polar compounds. The spots were observed to have moved from its spots as they all did not travel straight some were going to the