High performance liquid chromatography

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  • Papaveris Pericarpium

    the central nervous system.4 However, due to the relatively ubiquitous use of Papaveris Pericarpium in traditional Chinese medicines, its clinic safety has attractive significant attention for MOR and COD related toxicities. For example, MOR has a high potential for addiction, and its tolerability develops rapidly by frequent administration. In addition, MOR and COD have several severe side effects, such as nausea and vomiting, pruritus, constipation, urinary retention, respiratory depression,…

    Words: 768 - Pages: 4
  • Gas Chromatography Method

    Several methods have been described in the literature for the determination of MNT in its pharmaceutical dosage forms or in biological samples. Examples of these methods in biological samples are gas chromatography – mass spectrometry (GC-MS) [18] in plasma and urine of rats and human while GC-FID [19] was used for MNT analysis in human urine. Also, MNT in mixture with other drugs have been assayed in human plasma using GC-FID [20]. In pharmaceutical preparations…

    Words: 945 - Pages: 4
  • Essay On Mandrax

    drug and according to health 24 has the highest per capita Mandrax abuse in the world. When dugs are manufactured illegally the main aim, is produce a substance that is highly addictive, this creates dependence which at the end keeps the drug at a high consumer demand. This means that they are lots of impurities that are added to the drug to increase an individual’s dependency to the drug. In South Africa , and other countries in Asia that are still manufacturing Mandrax, the main impurity that…

    Words: 1110 - Pages: 5
  • Purification And Characterization Of Biosurfactants Case Study

    emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40 mL of dichloromethane in a separating funnel and shaken vigorously and the allowed…

    Words: 759 - Pages: 4
  • Acetaminophen Analysis

    women and children, acetaminophen is available over the counter in many forms. When consumed over long periods of time or in high dosages, acetaminophen has serious side effects including liver damage and other organ damages, therefore an effective quality control method of analysis. Here a method using cyclic voltammetry to analyze the concentration of acetaminophen in a liquid suspension Publix brand children’s pain reliever was developed and validated. A linear relationship from 0.79 ppt to…

    Words: 951 - Pages: 4
  • Reverse Micellar Extraction Of L-Asparaginase Case Study

    1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

    Words: 1432 - Pages: 6
  • Nt13101 Unit 3 Research Project

    to test manufacturing efficiency of two different types of polycaprolactone (PCL) with different average molecular weight. I concluded that the low molecular weight PCL is more convenient for mold-casting fabrication, due to low viscosity, while the high molecular weight PCL has an advantage of being mechanically stronger than the brittle…

    Words: 824 - Pages: 4
  • Neurocognitive Assessment: Neurocognitive Analysis

    part of an active collaboration with Dr. Tilla S. Worgall and/or in the Laboratory of Toxicology and HPLC of the Department of Clinical Laboratories, Pontificia Universidad Católica de Chile. Sphingolipid levels will be quantified by high performance liquid chromatography-triple quadrupole-tandem mass spectrometry (HPLC-MS/MS) on an Agilent 1200 HPLC system, equipped with an Agilent C18 column as described in protocol implemented and validated by our group2. Sphingolipid profiles will include…

    Words: 308 - Pages: 2
  • Uridine Synthesis

    This experiment monitored the synthesis of uridine from the deamination of cytidine through separating the contents of the reaction mixture using HPLC (High-performance liquid chromatography) at various time intervals since the start of the reaction. Cytidine and uridine standards are ran and the output of the HPLC will show two peaks based on the retention times of each compound. Identified the peak with the retention time of 2.422 minutes is cytidine and uridine has a retention time of 2.716…

    Words: 489 - Pages: 2
  • Protein Purification Practical Report

    Protein Purification Practical Report Student Name : Noel Murphy Date : 28 February 2015 UF/DF Practical Question Report Ultrafiltration (UF) and Diafiltration (DF) steps are carried out prior to every chromatography step and post the final polishing chromatography step. These UF and DF steps are vitally important, the UF step concentrates the protein product into a smaller volume of buffer, while the DF step alters the conditions the protein product is sitting in i.e. Buffer exchange.…

    Words: 3192 - Pages: 13
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