High performance liquid chromatography

Gas chromatography (GC) is a chromatography technique where the separation of individual components (called analytes) from a sample relies on their differing distribution between the mobile phase and a stationary phase. The mobile phase is what carries the analyte (components being analysed) through the stationary phase and in GC, it is an inert gas, (usually helium or nitrogen). The gas must be inert so it will not react with the samples to give a false reading. The stationary phase is the…

interactions [X16]. The more recent inlet techniques (Lasterspray ionization inlet [LSII], matrix-assisted ionization inlet [MAII], and solvent-assisted ionization inlet [SAII]) are sensitive, useful at both high and low mass range and can be used at ambient pressure or with a vacuum (With liquid or tissue specimens too) [X16]. These inlet techniques are functionally similar to ESI in the spectra they produce, these techniques also inherit the delicate treatment of proteins from ESI too…

Kate Byrne DT299/4 CHEM 4128 Applied Physical & Analytical Chemistry Assignment Liquid Chromatography High Resolution Mass Spectrometry Hyphenated Techniques The term “hyphenated technique” was introduced by Hirschfeld in 1980. It refers to the combination of a chromatographic separation technique and a spectroscopic detection technique to form a new, more efficent instrument. The technique which will be discussed in this case is LC-MS. The aim of combining both techniques is to purify and…

Laboratory, I successfully reverse engineered three different Henkel products and completed a competitive analysis of an equivalent product. Throughout the research process, I used many different analytical techniques including High Performance Liquid Chromatography and Gas chromatography–mass spectrometry to quantitatively and qualitatively define the components in the products, documenting the processes and results as the research was actively conducted. I am currently interested in…

psychoactive substances” (NPS) online and from friends, without an understanding of what they are actually taking. Deaths due to designer drugs happen, and often the victims are unsuspecting, uninformed young adults just looking to have fun and get high. When these new, previously unrecognized drugs are synthesized and widely distributed to young people across the country, affecting their safety, it constitutes a threat to the health of the public. In the case of a tragic drug overdose/drug…

(II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC, capillary electrophoresis and super-critical fluid chromatography[2,3], in urine by gas chromatography- mass spectrometry[4] and, simultaneously with its active metabolite in biological fluids, by HPLC[5- 10]. Amlodipine Besylate (ADB) )[FIG.1.B], chemically,…

Natural products either as pure compounds or as standardized extracts provide unlimited chances for new drug discoveries because of the unmatched availability of chemical diversity (Cos et al., 2006). According to the World Health Organization (WHO), more than 80% of the world's population trusts on traditional medicine for their primary healthcare needs. Plants used for traditional medicine contain a varied range of compounds that can be used to treat chronic as well as infectious diseases…

6. Casey DM. and Scheer EC. in 1993 reported the use of several surface conditioning agents on temporary soft lining material. The three different surface materials were treated with Poly ethyl methacrylate monomer, Minute-Stain glaze, or mono-poly glaze. These different samples were compared after 30 days. Samples were analyzed with the use of scanning electron microscope. Initially the surface of all samples was intact. Fewer irregularities were seen in monopoly and Minute-Stain glaze treated…

Chapter 1: Introduction 1.1 Nature of the Dissertation Identification, quantification and analysis Amoxicillin and Flucloxacillin Sugar free antibiotics which are manufactured at Athlone Laboratories by high liquid fluid chromatography (HPLC). The project also involves Method Transfer validation by analysis Amoxicillin Sugar Free for the new project of GSK Mayenne France, this involved Identification, quantification and analysis of carrying Amoxicillin Trihydrate API and Sodium Benzoate in…

Protein-protein interaction can be defined as the interactions between different protein molecules within the cellular organization leading to formation of large protein complex which is modulated by non-covalent interactions such as hydrophobic interaction, hydrogen bond, van der Waals interactions etc. Protein-protein interaction plays an important role in functioning of body’s significant mechanism such as replication, transcription, translation, signal transduction, cell cycle, etc. Thus,…