resistance gene will also be employed in this experiment. Ampicillin will then be added to the transformed bacteria in order to obtain the successfully transformed bacteria. By adding this engineered PSII antenna, the efficiency of light capture is increased. This experimental group will also be placed in nitrogen starved conditions and the photosynthetic (light) conditions will be identical to those in the control group. These mutant…
Question 1A: Compare and contrast the general structure of a virus and a bacterium. Bacteria and viruses both do not contain a DNA enclosing nucleus; however, these organisms differ greatly in the complexity of their structures. Generally a bacterium is encompassed within a semi-rigid cell wall made of peptidoglycan, which provides structural support. It is classified as gram positive if its cell wall is thick and stains purple when tested for identification. If its cell wall is thin and stains…
Identifying a bacteria based off of physical characteristics is almost impossible due to the commonality of shapes and physical features. Because of this, a wide range of biochemical tests were preformed in order to better identify the bacteria. Being able to identify an unknown was the most interesting part of the microbiology lab this semester, as well as, being able to preform the various tests. Proper aseptic technique was used throughout the semester and followed during all biochemical…
Bacteria are commonly associated with pathogenicity. There are multiple factors that can contribute to bacteria being pathogenic. One factor is sporulation. Sporulation is a specialized function that select bacteria undergo when they are exposed to harsh environmental conditions (Fimlaid 2015). Clostridium difficile, Clostridium botulism and Bacillus anthracis are three examples of sporulating bacteria that cause serious human disease. In a 2011 study, the Centers for Disease Control and…
Bacteria are coming from the Greek word bakteria, meaning little stick, even though the first observed bacteria was in a rod shape, bacteria can come in many forms, spirals, spherical in shape. In 1928 a British scientist named Frederick Griffith discovered that previously harmless bacteria could change their own nature and become capable of causing disease (Black, 2012) What exactly then is a bacteria? Is a microscopic living one cell organism, consisting of a cytoplasm enclosed in a cell…
All bacteria is considered unknown and has specific characteristics that can give clue on what the bacteria species is its important to be able to identify bacteria because bacteria can potentially cause disease and illness also if bacteria causes disease a cure or treatment would need to be developed to save a life, but to be able to identify bacteria it takes several direct steps which include culturing, staining, and microcopy. However, bacteria could not be identified with those steps alone…
types of bacteria inhabiting the sample of forest soil tested. Methods: Many steps were taken to isolate and identify the different types…
Introduction Our world is composed of many bacteria that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods learned in the laboratory to determine our unknown bacteria. Bacteria can have different features, shapes, and or arrangements that help microbiologist differentiate them. Bacteria can be classified as Gram positive or negative (difference in cell wall), with the help of the Gam stain (“Gram…
represents the ecology of life with bacteria. To test how the ecology of life works, we measured and reviewed the growth of bacteria after washing one’s hands at different intervals of time. After washing our hands four times in 30 second intervals, we wiped a finger on a different segment of an Agar plate where our bacteria would have a strong environment to grow and develop. To be sure that each individual whose’ hands would be tested had a source of bacteria on them, we had the freedom of…
Bacteria also known as prokaryotes are very ancient single-celled organisms; fossils show that they were widespread 1.5 billion years ago. Today bacteria make up the vast majority of prokaryotes on earth due to the unfavorable ability to adapt quickly to the surrounding environment. Bacteria have the capabilities to survive in areas of high heat, extreme cold, or even very acidic or alkaline conditions. This ability is used frequently to fend off the effects of antibiotics and antibacterial;…