Bacteriology

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for Type up labels for each sample using the salon code, bowl number, and location on bowl Cut out each label Get materials needed including gloves, sterile swabs, saline, sterile aluminum foil, approval letter, tape, and a pen Go to each pedicure spa Receive approval from the pedicure spas you plan to test by obtaining a signature of an employee Acquire sterile swabs Drop multiple drops of saline onto the sterile swab to ensure the attachment of bacteria in the salons. Go to the pedicure bowl and swab the bottom of the bowl around and in the drain Wrap the sample individually in sterile foil Label the sample by salon, bowl number, and location in bowl using the salon key Put the sample in a sterile bag Acquire another sterile swab Drop multiple drops of saline onto the sterile swabs to ensure attachment of bacteria onto the swab Go to the pedicure bowl and swab side of the bowl by moving the swab from side to side on either side of the bowl Wrap the sample individually in sterile foil…

A. Description of the Study Area The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert…

The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it…

The possibilities of two unknown microorganisms lead to phenylalanine deaminase test. The detection of phenylalanine deaminase enzyme was carried out by adding ferric chloride to phenyaline slant. When ferric chloride was added to phenylalanine deaminase tube, it turned into army green color( positive result) which indicated that unknown microorganism contains phenylalanine deaminase enzyme. On the other hand, no color change in the slant indicates negative result for this test. The choices…

During the first lab period, The gram stain show that the unknown bacteria was a gram neative rod shaped microbe. After this step was established, this next part was to select the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For…

Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…

Identifying a bacteria based off of physical characteristics is almost impossible due to the commonality of shapes and physical features. Because of this, a wide range of biochemical tests were preformed in order to better identify the bacteria. Being able to identify an unknown was the most interesting part of the microbiology lab this semester, as well as, being able to preform the various tests. Proper aseptic technique was used throughout the semester and followed during all biochemical…

Arooj Kamran 10/23/16 Lab Report 818 Comparing Disinfectants Lab Abstract The purpose of this lab is to test how well disinfectants can control the growth of bacteria. Three Petri dishes were contained with bacteria and different types of hand sanitizers in each. This experiment tests…

In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…