Essay On Triple Sugar Iron Test

Better Essays
A. Description of the Study Area
The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert Auditorium. The laboratory consists of several laboratory apparatus and most especially microscopes, which is greatly needed for the experimentation. Beverage samples will be collected from University Canteen, located in the University Activity Center, near the Gusaling Villegas Right Wing and PLM Pride Hall.

B.
…show more content…
Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards.
4. Oxidation-Fermentation Test (OF Test) In OF slants, a loopful of culture will be stabbed halfway to the bottom of the slants. One slant will be placed with mineral oil to create an anaerobic environment. The OF slants will be incubated for 48 hours at 35°C, and the results will be recorded and photographed.
5. Methyl Red and Voges-Proskauer Test
A pure culture will be inoculated in a Methyl Red-Voges Proskauer Broth tubes. The tubes will be then incubated at 35°C for 48 hours. One tube will be placed with five drops of methyl red reagent, and the other with Barrit’s reagent. Methyl Red test is used for the indication of fermenting ability of bacteria, producing mixed acid. Voges-Proskauer test is used to detect the production of acetoin, also known as 2,3-butanediol.
6. Indole – Motility

Related Documents

  • Decent Essays

    Camphene Synthesis

    • 1013 Words
    • 5 Pages

    For the first synthesis reaction, camphene was converted to isobornyl acetate. In the fume hood, a sample of 1.36 g of camphene was added to a 25-mL round-bottomed flask along with a stir bar. To dissolve the camphene, 3.0 mL of glacial acetic acid was added into the flask. The flask was fastened onto a ring stand and immersed in a water bath that was on top of a stir plate. A thermometer wire was placed in the water bath to monitor the temperature throughout the experiment.…

    • 1013 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    After about 5 minutes, turn off the aspirator system until acid fumes can be formed only in the top Exhaust system. Continue the digestion process until the formation of green clear sample in tube. Upon completion of the digestive process, move the rack from tubes to refrigerate the tube vertically for 10 to 20 minutes. After that, add 75 mL of distilled water carefully into the tube which has been cooled followed by the 50 mL of 40% sodium hydroxide, NaOH. In order to prepare the distillate samples, 25 mL of 4% boric acid with 10 drops Bromocresol green indicator are added to recipient solution in a 250 mL conical flask and subsequently placed into the Kjeltec 2100 Distillation unit (Gerhardt, Germany).…

    • 1632 Words
    • 7 Pages
    Decent Essays
  • Decent Essays

    Elisa Research Paper

    • 1444 Words
    • 6 Pages

    Before placing anything in the biosafety cabinet, spray the BSC with 70% ethanol as well as any material put inside the BSC. Use a sterile 500 mL bottle and label it with the media name, contents, expiry date, preparation date, and preparer’s name. Use a pipette to add 450 mL of RPMI basal media into the sterile bottle. Then add 50 mL of FBS into the sterile bottle and stir for two minutes to allow the FBS to be thoroughly mixed. Next, add 5 mL of 1X Pen-Strep and 5 mL of L-Glutamine.…

    • 1444 Words
    • 6 Pages
    Decent Essays
  • Decent Essays

    The examination of synergistic and decreasing effects of extracts on antibiotic is done under inhibitory concentrations (sub-MIC). Concentration of the extracts under inhibitory state to the Mueller-Hinton agar is added with a ratio of 1 to 2 (1 unit extract and 2 unit Mueller-Hinton Agar medium) were used as plate test. Bacterial suspension, with a concentration of 0.5 McFarland (108×1.5 CFU/ml), is cultured on Mueller-Hinton Agar medium containing inhibitory concentrations of the extract in a grass-like state. Antibiotic discs was placed on the surface of the medium. The disc containing solvent is determined as negative control and a disc with extract and an inhibitory concentration (sub-Mic) was recorded as positive control.…

    • 831 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    A series of test tubes (5 in number) were filled with 5 ml of nutrient broth, autoclaved and supplemented with varying concentration of plant extracts. 0.05 ml of bacterial pathogens was inoculated and the test tubes were incubated overnight. Next day, the growths of bacterial pathogens in presence of plant extracts were recorded by taking an un-inoculated nutrient broth as reference. The minimum concentration in the media which was able to check the growth of pathogens was considered as MIC of the compound against that…

    • 1032 Words
    • 5 Pages
    Decent Essays
  • Decent Essays

    The proteins from each solution will then be precipitated by ethanol and suspended in 2-D cell lysis buffer (). Next, the proteins from the healthy volunteers and the sick volunteers will be stained with CyDye Cy3 and Cy5, respectively, and combined into one solution. The resulting solution will then be subjected to 2-dimensional gel electrophoresis. This will be done by transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting it to a constant electric current (145mA). After the gel has been allowed to run for about an hour, the isoelectric tube will be rinsed with running buffer and set aside.…

    • 1330 Words
    • 6 Pages
    Decent Essays
  • Decent Essays

    Bacteria Lab Report

    • 343 Words
    • 2 Pages

    The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder. Dissolve completely the nutrient broth powder in beaker containing 200 ml of distilled or the deionise water.…

    • 343 Words
    • 2 Pages
    Decent Essays
  • Decent Essays

    The temperature is set at 60 °C for 2 days for aging. After that, the samples were cleaned with acetone, and then dried at room temperature for later performed in heat treatment process. In this process, the samples will be dried in the furnace using a temperature of 300 °C for 30 minutes. Finally, the sample is ready to the further characterization and measurement for sensor performance testing…

    • 334 Words
    • 2 Pages
    Decent Essays
  • Decent Essays

    Incubation of Macrophage with MSCs 100 µl of MSC suspension (2 × 105 cell/ml) was added to each well of 96-well microplates containing macrophages and incubated for 4 h at 37°C in a moist atmosphere of 5% CO2. 2.5 Phagocytic assay by neutral red The basic phagocytic ability of macrophage (non-opsonic dependent phagocytosis) was measured by neutral red uptake. After MSCs and Macrophages were co-cultured for 2 h, 200 µl neutral red solutions (dissolved in 10 mmol/L PBS with the concentration of 0.075%) was added and incubated for 1h. The supernatant was discarded and the cells in 96-well plates were washed with PBS twice to remove the neutral red that was not phagocytized by macrophages. Then cell lysate (ethanol and 0/01 % acetic acid at the ratio of 1∶1, 200 µl/well) was added to lyse cells.…

    • 796 Words
    • 4 Pages
    Decent Essays
  • Decent Essays

    Essay On Biochemical Test

    • 1565 Words
    • 6 Pages

    The MAC agar plate that was stored in the incubator at 37°C for about 48 hours until the next lab time presented colonies in a dark red/pink color hinted that the microbe is a lactose fermenter(117). After 48 hours of incubation at a 37°C, BCP…

    • 1565 Words
    • 6 Pages
    Decent Essays