6. Three Nutrient Agar Plate (NAP) were contaminated as follow to illustrate that there are microorganism all around us and to demonstrate the necessity for proper aseptic technique. a) The lid of the first agar plate was removed and the exposed agar was placed in the building on the laboratory rack for 3 hours. The lid was replaced and labelled as “air”. It was then incubated at room temperature. b) A second Petri plate was divided in half using a marker. Two cotton sterile swab were moisten in sterile water. One of the swab was rubbed over one of the stool in the laboratory and the other swab was rubbed over the sink in the laboratory. Both swabs were used to inoculate the each of the halves of the second Petri plate respectively. The plate…
Nutrient agar plate Materials used in experiment 1.1B: Nutrient agar plate, Sterile cotton swab In experiment 1.1B a nutrient agar plate was opened and left in an arbitrary spot in the laboratory and left untouched for three hours, at the end of the lab it was collected and incubated at . In part B a swab/swab rinse method was preformed in order to inoculate the nutrient agar plate. A sterile cotton swab was taken out of its packaging, an aseptically moistened with sterile saline. The bottom of…
1. 4 agar plates were poured several days ahead in preparation for this experiment. They were stored in a Ziploc bag in the refrigerator until ready to use. 2. To perform this experiment are the: cultures of E. coli, S. cerevisiae, and S. epidermidis, three plastic inoculation loops, candle and lighter, three poured nutrient agar plates, a disposable cup, alcohol, paper towels, bleach, a permanent marker, gloves, mask, apron, and goggles. 3. Be sure to wash hands carefully with disinfectant…
Starch Hydrolysis: Materials: - Starch Agar Plate - Unknown organism # 40 - Iodine - Inoculating Loop Procedure: A Starch agar plate made up of nutrient agar and starch is used to detect amylase activity. One loopful of the unknown organism #40 was inoculated in a straight line onto the starch agar plate. Inoculating tool was sterilized with heat before and after inoculation. The inoculated starch plate was then incubated at 37℃ for 24-48 hours. After incubation period the starch agar plate…
The gas generation is due to E. coli metabolic processes that generates acid and changing the turbidity. 2. The Membrane Filtration Method did work as well. MF method can replace the MNP test for microbiological analysis of water sample. After the incubation of the mEndo plate for 24 hours it is perceptible the grown of E. coli colonies across the plate. The results of this method are shown in the figure 4. As it was used mEndo, just the E. coli colonies form bright green colonies with a…
the eight chosen S. aureus isolates (four MRSA+VRSA and four MRSA+VSSA) were analyzed through determination of their minimum inhibitory concentrations (MIC) values, minimum bactericidal concentrations (MBC) values, and MBC/MIC ratio by broth micro-dilution method using 96-well micro-titer plates. To each 50 μL of the antibacterial agent dilution, 50 μL of adjusted bacterial concentration inoculum (5 × 105 CFU/mL) were added in the 96-well micro-titer plates, the growth control wells contained MH…
Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also…
Scientists use different types of nutrient agar media to help with the study of microorganisms. The purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable…
Once completed label the bottom of the blood agar plate (not the lid) with the details of sampling area e.g. Name, Date, Location, Sampling Area. Complete the procedure 2 more times in either a clinical or a non-clinical area. Part 2: Cross-Infection Control Part A Method: Sit at a bench with elbows on the bench, head resting upon the hands and poised directly over an open blood agar plate. Recite (with enthusiasm!) the following incantations 10 times, emphasising the labial and glottal…
Assessment of enzyme producing efficiencies of endophytic fungi Extracellular enzymes assay was conducted to investigate the production of enzymes by the endophytic fungi Amylase activity Amylase enzyme was assessed by growing the fungi on glucose yeast extract peptone (GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium…