Agar plate

a MacConkey agar plate, and then streaked once more on a separate MacConkey agar plate. Two plates were used in an effort to ensure that isolated colonies were produced. The aseptic technique was also used, and the loop was sterilized in the bactoincinerator after each streak for the same reason. The two MacConkey agar plates were placed in the incubator (35oC) and then retrieved 24 hours later. Upon retrieval, the two plates were gathered and isolated growth was seen on both plates. However,…

microorganisms. One of the microorganisms is gram positive which can be separate from the gram negative using the four-way streak isolation from the lab manual. The purpose of the streak isolation is to obtain single colonies on a Columbia CNA agar plate. C-CNA agar is a type of…

Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…

Then trypticase soy agar (TSA) was poured into into six groups of 60 Petri dishes. Sixty different agar dishes were labeled based on the antibiotic used. Then the dishes were left to dry and solidify at room temperature for hour to rid of moisture. After an hour, the dishes were placed in a refrigerator at three degrees Celsius. The agar plates were monitored every few hours to make sure that the plates did not accumulate moisture. If there was visible condensation on the agar plate, they…

3. I chose the plate with dilution factor 10-7 to count. This was the only plate that yielded an appropriate CFU, therefore, it was the sample with the highest countable colonies. The plate with the dilution of 10-6 yielded Too Many To Count (>300) while the plates with dilution 10-8 and 10-9 yielded Too Few To Count (<30). 4.If I were to do a total count on this sample, as opposed to a viable count, the total count would be higher because it accounts for all cells, not just visible and…

helped lead to the identification of the unknown. For this procedure, you grab a Blood Agar plate, and then streak the plate with a flamed loop containing your unknown. After the plate is streaked, you will then ask your instructor to place antibacterial discs throughout the plate. These antibacterial discs, after growth, will show the zone of inhibition which will then lead to your results. You leave the plates for a couple days in order to later on see some growth. My results were…

Discussion: Each test is a pertinent contribution to drawing an educated conclusion. After performing the gram staining and observing which unknown culture was gram positive, a catalase test was performed. The catalase test allows lab workers to differentiate Staphylococci and Streptococci genus. The test was performed only on the gram-positive culture. Bacteria that respire by the use of oxygen, otherwise known as aerobic respiration, produce enzyme or “catalase”. This protects the bacteria…

used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment lactose. A Gram stain was then performed to…

Methods 1. Treatment of barley with endophyte. • Five types of Barley were treated with the endophyte Pseudomonas fluorescens L321. • They were innoculated with the seed coating mixture ( 4% sodium alginate and 1% skimmed milk powder) without the bacteria. The bacteria were grown up overnight in LB broth supplemented with 3% trehalose (400ml per strain) , centrifuged and then added and then resuspended in the coating mixture. • 30ml of inoculant mixture was then added to 1kg of seed in a…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…