Petri dish

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    will be transferred to a clean glass slide and will be smeared and heat-fixed. The slide will be flooded with crystal violet for 1 minute and rinsing with distilled water. Next, the slide will be flooded with iodine solution for 1 minute, and rinsed with distilled water. It is then decolorized with alcohol and will be drained, and counterstained with safranin for 30 seconds. The slide will be rinsed and excess stain will be wiped out, and then air dry. Finally, the slide will be observed under microscope to determine the characteristic of the bacteria, and will be photographed using a cell phone camera. 2.Catalase Test Catalase test will be performed also, using protocol of Reiner (2013). A clean glass slide will be placed on a sterile petri dish. A single colony from a 18 to 25-hour culture will be inoculated and will be placed on a glass slide, because bacteria loses its catalase activity with age. A drop of 3% Hydrogen peroxide will be placed directly onto the organism and will be covered immediately. Results will be recorded and photographed. 3. Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards. 4. Oxidation-Fermentation Test (OF Test) In OF slants, a…

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    Sordaria Lab Report

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    In order to obtain accurate numbers from the now grown-out Sordaria samples, a small procedure was followed. First, a sample of fungi was taken from the border of a WT and TT section in the Petri dish. A slide was prepared by placing one drop of water on the middle of it, the Sordaria sample on top, and a slide coverslip on top of the sample. The eraser of a pencil was used to lightly press on the coverslip in order to release the asci so that they can be seen. Under a microscope, a multitude of…

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    Laurier is a school of over 150 students. It is important that the building is kept adequately clean to prevent students from getting sick. Some places in the school that are overlooked by the caretakers, might have the most bacteria. Which spot in the school is the dirtiest? Considering the cleanliness of fish, the fish tank could very well be the dirtiest spot in the school. The petri dish was sanitized and covered in the agar. The cotton swabs used to swab bacterial samples were dampened…

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    Bacteriophage Experiment

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    containing the phage is known as supernatant.The supernatant was filtered through a .22 micron filter in order to extract phages. Since bacteria and other particles tend to be bigger than .22 microns, they would not fit through the filter, thus allowing only phages through. Mycobacterium smegmatis was then added to the supernatant in the amount of .5 mL. It was allowed to incubate for 5 days. After incubating, 1.4 mL of the enriched filtrate was pipetted into a microcentrifuge tube. This was…

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    Petri Dishes Lab Report

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    The ten Petri dishes that will exclusively not contain an antibiotic in bacteria culture serve as the control. There will be ten trials for the control and each level of IV. The experiment began by cleaning the work area and sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into into six groups of 60 Petri dishes. Sixty different agar dishes were labeled based on the antibiotic used. Then the dishes were left to dry and solidify at room temperature for hour to rid of…

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    Photosynthesis Lab

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    Methods: To start this experiment place four seeds in four separate petri dishes with a damp paper towel. Once the seed germinates and the plant has begun to sprout, move the seeds to four separate pots, and add soil. Once the plants have begun to sprout take the measurement of each plant and record your data for day zero. Next, take three glass containers of greatly varying volumes (41,4185cm3, 2,250cm3, and 602cm3 were used). Before the plants are placed underneath these containers, give…

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    2 antimicrobial agents are against 2 different strains of bacteria within a cultured environment. Method: Apparatus: • Small Bottle of S.Aureus bacteria in Nutrient Broth • Small Bottle of E.coli bacteria in Nutrient Broth • 4 Nutrient Agar plates (2 for each bacteria) • A P200 Gilson pipette • A box of sterile P200 yellow tips • Sterile spreaders • Latex gloves • Sterile filter disks • Forceps • Test samples of antimicrobial agents (Milton and Dettol) • Sterile distilled water • Virkon …

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    Oral Microbiome Essay

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    Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

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    Escherichia Coli

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    Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…

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    Vocabulary: Study Guide

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    loop? a. After sterilizing the loop over an open flame, depending if the bacteria is in a liquid or solid form, we dip the loop inside the sample test tube and dip the inoculated loop straight down in the tube. If the sample is a solid the sterilized loop should barely touch the culture. 4. Why do we have to wait for the loop to cool before collecting a sample? a. We have to wait for the loop to cool before collecting samples because we may kill the bacteria or melt the agar jelly that is…

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