Escherichia coli

Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient agar plate for differential the isolate colonies presence. IV. To learn how to know whether the culture is a selective or differential media. The culture bacteria that will be using are E. coli, Bacillus subtilis, staphylococcus aureus and Proteus vulgaris. V. To learn how to find the extracellular enzyme production by bacteria. The culture bacteria that will be using are E. coli, Bacillus subtilis, and staphylococcus aureus. Result i. The two…

of K12 strain Escherichia Coli? Which household cleaning product (hydrogen peroxide, Pine-sol, Clorox Bleach, Mr Clean antibacterial liquid cleaner) is the best inhibitor of K12 Escherichia coli (E. coli) growth? The manipulated variable was the cleaning product or disinfectant on used of the Whatman antibiotic assay paper disc. The dependent variable is the zone of inhibition of the prokaryotic bacteria E. coli. In topic one IB requires that we learn about Eubacteria, traditional bacteria…

The spectrophotometer analysis of Escherichia coli growth during heat application The purpose of this experiment is to determine the rate of Escherichia coli (E. coli) growth over 100 minutes in a liquid growth medium (control), and to determine the rate of E. coli growth over 100 minutes in a heated liquid growth medium. The prediction is that the control E. coli culture will grow at a faster rate exponentially over 100 minutes than the experimental bacteria culture. Consequently due to heat…

Classification Escherichia coli is a bacterium that is mostly found in humans and other animals. It is a unicellular micro-organism, making its Domain and Kingdom Bacteria. E. coli’s Phylum is Proteobacteria because it is a gram-negative bacterium. It is also in the Phylum Proteobacteria because of the lipopolysaccharides that protect the cell membrane. E. coli is a facultative anaerobic G- bacterium, making its Class Gamma Proteobacteria. Because of its rod-shape, E. coli’s Order is…

Bacterial Transformation with Escherichia coli Byanka Delgado BIO 181 Lab Thursday 1:30-4:15 Arizona State University Abstract The major objectives of the Bacterial Transformation Lab were to observe standard bacterial growth under various conditions including the transformation of bacteria; to understand how the process of transformation occurs, as well as the biological results and consequences that come of transformation. In order to correctly perform this lab, plasmids, agar plates,…

Escherichia coli is a gram negative bacteria with pathogenic potential to both humans and animals causing three general clinical syndromes: (1) enteric/diarrhoeal disease, (2) urinary tract infections (UTIs) and (3) sepsis/meningitis (Kaper et al., 2004). However, this bacteria uses different virulence factors to cause the above-mentioned clinical syndromes (Croxen and Finlay, 2010; Kaper et al., 2004). The E. coli producing pore forming toxin α-hemolysin causing bacteraemia/sepsis causes…

Escherichia coli and Me The disease/infection that I have chosen is the urinary tract infection, commonly known as the UTI. Depending on where the infection occurs, there are two specific names. If the infection occurs in the lower part, like in your bladder, it is called Cystitis. If the infection travels up towards your kidneys, it is called Pyelonephritis (Umm/Health). The microbe that causes this infection is Escherichia coli, more commonly known as E. coli. It is found commonly in…

Unknown Organism Report I have identified my Unknown Organism #1 as Escherichia coli, or E. coli. My Unknown Organism is an anaerobic gram-negative, unicellular eubacteria. It belongs to the genus Escherichia, which is named after Theodor Escherich, a German pediatrician. E. coli is usually found in the colon of mammals, explaining the name “coli” meaning from the colon. It’s classified in the order of enterobacteriales, and the family of enterobacteriaceae. It is the most abundant bacteria in…

Let’s start the introduction by talking about our main target, Escherichia coli or how it is mostly unknown, E.coli, are a diverse and large group of bacteria found food, the environment, and in the intestinal tracts of humans and animals were it normally inhabits and it can also cause infection in other parts of the body, especially the urinary tract. Its name was given by a German physician called Theodor Escherich and it is the most common member of the genus Escherichia. E.coli is “a…

The identification of Escherichia coli as the unknown was concluded by a string of tests and observations such as gram-staining, shape, motility, oxidation, fermentation and citrate tests. This began with gram-staining the organism. After viewing beneath a light microscope, the unknown showed pink rods, proving to be gram-negative. The observation of motility was made by viewing the bacteria moving towards a specific direction. The next tests utilized in determining the unknown was oxidation…