Escherichia coli

bacteria and as well against fungi. Antimicrobial activity of the drug Tal Sindoor can be tested in 2 methods i.e. Gradient plate technique and Kirby-bauer method. Tal Sindoor has an effective anti-microbial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus mutans and Candida albicans. But K. pneumoniae and A. Baumannii are resistant to Tal Sindoor like they are with other…

A loopful of Staphylococcus epidermidis and Escherichia coli were separately and aseptically transferred to the first centrifuge tube, which was labeled “A”. A loopful of the mixture from the first centrifuge was transferred to the second tube, which was labeled “B”. This process was repeated for the third…

bacteria that are used daily in the medical or research field. Escherichia coli is a bacteria that is found in the intestines that is not harmful and is used by many laboratories where they perform many experiments with it. Researchers value Escherichia coli for its genetic simplicity (it has about 4400 genes, versus the 30,000 in a human cell) and for its fast growth (Feltman). One of the research that is very popular with Escherichia coli is bacteria transformation. Transformation, in biology,…

coli, in a water source is an indication of the presence human or animal waste matter in the water. These bacteria are a type of fecal coliform bacteria found in the intestines of humans and animals. Drinking water that contains E. coli bacteria may cause diarrhea, abdominal cramps, headaches and nausea. Young children and elderly people may be more susceptible to the adverse effects of E. coli because their immune systems are delicate. Polluted…

Mannitol Salt Agar). This agar has a very high concentration of salt which is a good environment for the growth of Staphylococcus bacteria only (Beaver, L et al. Mannitol Salt Agar). The next agar that is used is EMB. EMB is a good agar for recognizing E. coli (Beaver, L et al. Eosin Methylene Blue Agar) E.coli ferments a lot of sugars which reacts really well with the EMB and produces a very noticeable green color (Beaver, L et al. Eosin Methylene Blue Agar). The final agar that is used is the…

suffering from kidney failure. A urinary tract infection is caused by organisms/microbes including bacteria, fungi, and viruses that affect the urethra and could spread to the bladder and kidneys. The majority of UTIs are caused by the bacterium Escherichia coli (E-coli), most live in the bowel and others like Chlamydia that affect the reproductive system. There are…

Hypothesis: That Domestos is more effective in killing both the Escherichia coli and M bacteria than Dettol Pine o Clean and White Vinegar. Pine O Clean is more effective on gram-positive bacteria than gram negative bacteria, whereas Domestos has the ability to effectively destroy both gram negative and positive bacteria…

activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram -ve bacteria, Yeast and Filamentous Fungus with MICs 150, 150, 75, 312, 625 and 625 µg/mL against (Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 3555, Escherichia coli ATCC 23282 Pseudomonas aeruginosa ATCC 10145, Candida albicans IMRU…

This was avoided by introducing the ampicillin strain into the E.coli cells which made them ampicillin resistant. Thus by transfection of the DNA, E. coli was able to take up the strain into its genome and recombination occurred as the cell became ampicillin resistant. That is why colonies were able to form on the agar plates. All the experimental plates had colonies including the agar plate of the enzyme…

The purpose of this experiment is to determine is to be able to successfully transform Escherichia coli Bacteria, and make it ampicillin resistant when exposed to an extracellular plasmid of DNA. It involves introducing the E. coli and allowing it to adapt to the DNA of a new environment. The E. coli must be placed in a tube, into an ice bath, causing the cell to shrink. From there it is transferred to a preheated bath, where the cell is heat shocked, allowing the plasmids ' to enter the cell at…