Escherichia coli

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    Oyindamola (Dee) Adedipe `Individual Reponses: Cow Discussion Our team’s discussion post focused on the benefits and risks associated with the addition of antibiotics and genetically modified E. coli to cattle feed the for the cow and its microbiome. Many of the benefits included in the posts pertained to the use of antibiotics to help improve the overall health of the cow. Antibiotics are administered to cows to help foster growth and prevent the occurrence of a disease (McEachran et. al.,…

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    Mary Farmer has Gram-positive cocci, Streptococcus aureus, and Brian Evans has Gram-negative bacilli, Escherichia coli. From this, we know that Culture A’s bacteria has a thick cell wall with no outer membrane and Culture B has a thin cell wall with an outer membrane. From the results of the Gram’s test, neither patient is infected with more than one strain and…

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    Phenylethyl Salt Agar

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    Introduction Life could not exist without microorganisms: plants depend on microbes to obtain soil and cows need microorganisms to digest cellulose. Microbes degrade waste and they live in our bodies to keep us healthy. Microorganisms are also used to make vaccines and antibiotics. However, some bacteria are known for causing diseases such as the common cold, AIDS, Herpes, and Chicken Pox. Knowing the identity of microbes is essential for knowing the causative agent of a disease in order to…

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    the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either ampicillin or not as well as with a ribosome known as arabinose. The E. coli without pGLO died in the ampicillin and the ones with pGLO survived…

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    Plasmid Synthesis

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    Transforming Genetic Material To E. Coli Using Plasmids Proteins are created within the body through a process known as central dogma. This process is the transcription of DNA to RNA and the translation of RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its…

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    Bionic Antibiotics VS. Bacteria Abstract: The bacteria lab preformed in class tested the effectiveness of antibiotics against bacteria. During the lab we tested the antibiotics penicillin, streptomycin and tetracycline against the bacteria Escherichia coli and Staphylococcus epidermis in order to determine which antibiotics fought which bacteria most successfully. In order to monitor the experiment, we kept a control sample in which no bacteria was present, only an antibiotic disk. After…

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    The fenicol groups of antibiotics are one of the generally utilized medications which are wanted to treat sicknesses relating to respiratory and digestive systems. These days, these gatherings of antibiotics are utilized as a part of veterinary prescription adequately. Fenicol gathering of anti-microbials incorporates a few regularly utilized medications like chloramphenicol and florfenicol. As of late, utilization of florfenicol has been expanded on account of its wellbeing in nature when…

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    Why Germs Are Bad

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    Germs are everywhere. Fortunately, most pose no risks to people. And those that do cause disease usually can be killed with antibiotic drugs. Sometimes, however, harmful bacteria evolve ways to “laugh at” antibiotics — survive as if the poisons were not even there. This so-called drug resistance make infections hard, if not impossible, to treat. How bacteria learn to resist killer drugs normally is invisible. But scientists have just unveiled a new tool that lets them watch it happen, right…

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    Gram-Negative Bacteria

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    Gram positive: First, the bacteria sample is placed on a glass slide and heated only to the point of rendering it innocuous in terms of being infectious to the handler. Next, the bacteria sample is treated with a gentian violet-iodine solution for up to sixty seconds. The slide is then gently rinsed under clean water and the Gram solution is applied, which is a mixture of iodine and potassium iodide diluted in water. This step triggers a reaction to the gentian violet compound. Initially, the…

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    Sexduction Lab

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    Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

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