Escherichia coli

the patient, unknown bacteria #618 was identified to be Escherichia coli. It is apart of the Enterobacteriae genus that according to Brown’s Microbiological Applications1 are gram-negative bacteria that usually live inside the intestines of animals and humans. The organism can also live in the vagina and urethra. The data collected after isolating the organism and executing a series of tests have confirmed previous observations of E. coli in past scientific history. For the majority of the…

These results lead to the identification of unknown 18X as Escherichia Coli. The Gram test was first performed on the provided culture of unknown 18X. To double-check the results, a second Gram test was performed from the incubated nutrient broth that was grown from a colony of the streak plate. It provided the same…

E coli Name: Course: Lecturer: Date: E coli Introduction Escherichia coli are bacteria although this is a large group of a scientific classification. It includes a diverse classification of these types of bacteria. E. coli occurs in different forms or strains with most of these strains being very harmless. However, there are other strains that are very harmful and can cause one to be sick. Some of these strains can cause diarrhea and in most cases urinary tract infections…

showed positive results indicated by the red color of the broth and the VP test showed negative results which were indicated by the lack of red color when adding the dye. The results of the MR/VP test lead me to the conclusion that my unknown was Escherichia coli but to be…

Escherichia coli, a bacteria with an optimal pH of between 6.5 to 7.0 to enhance its proliferation is very commonly used in laboratories and studies worldwide due to its low cost and high effectiveness and will therefore be the bacteria of choice for this investigation. It has been shown in many studies such as the article “HdeB chaperone activity is coupled to its intrinsic dynamic properties” very recently published in the prestigious magazine Nature that bacterial growth and overall…

sanitary practices recommended for the storage, preparation, display, and service of food. Table 3. Potential Hazards in pies Ingredients Potential Pathogens Process Control Fruits and vegetables Salmonella spp., Shigella spp., enterohemorrhagic Escherichia coli, Listeria monocytogenes, Bacillus cereus, Clostridium botulinum,…

Assignment #2: The Replication of DNA in Escherichia Coli Due Date: Feb 8, 2016 at 9:00 AM CST 1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure…

The Ampicillin Effect of Escherichia coli in a Beta Lactamase plate and non Beta Lactamase plate. Jimena Cárcamo Lab 4 Concepts in Biology 173 C October 6, 2014 INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing…

isolates and their frequency of isolation from dumpsites were Escherichia coli (33.3%), Klebsiella spp.(16.7%) ,Pseudomonas spp. (13.3%), Proteus spp.(10%), Streptococcus spp. (8.3%), Salmonella spp. (6.7%), Staphylococcus aureus (6.7%) and Enterobacter spp. (5.0%). The high presence of pathogenic bacteria from dumpsites is of serious public health concern and highly significant. Antibiotic susceptibility testing using the disc diffusion…

bacteria were streaked onto plates of Mannitol Salt agar, MacConkey agar, and Eosin Methylene Blue agar to examine their growth on the media. Two other unknowns were streaked on Chromogenic agar to determine coliform status and look for the presence of E. coli. All six species were streaked on nutrient agar as a control. The additional unknown #53 was isolated on a MacConkey agar plate, gram stained, and subjected to the IMViC series of tests. A more rigorous set of tests, known as the API 20E…