Staining

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  • Gram Staining In Microbiology

    it is the most important staining procedure in microbiology, it is used to distinguish between gram-positive and gram-negative organisms. The gram-positive cells stain purple, and the gram-negative stain a pinkish color. This staining is a differential stain that allows is used to identify the changes between organisms or differences within the same organism. Theory and Background: The Gram staining method was named after the Danish bacteriologist, Hans Christian Gram, in 1882. This method is now one of the most important staining procedures in microbiology. Steps of the Gram stain: Reagents: • Escherichia coli (slant culture) • Staphylococcus aureus (slant culture) • Crystal violet (primary stain) • Iodine solution (Gram’s iodine) • Decolorizer (ethanol) • Safranin (counterstain) • Water (in squirt bottle) Prepare a mixed smear using slant cultures: 1. Take two…

    Words: 1370 - Pages: 5
  • Essay On Gram Staining

    INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

    Words: 1139 - Pages: 5
  • Gram Staining Test

    Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. Gram-positive microbes contain a thick peptidoglycan with only one outer cell membrane, thus dying the microbes dark purple. Gram-negative microbes contain a thin peptidoglycan with an outer and inner cell membrane, dying the microbes pink when a gram stain test is done. Microbes A and C were gram- positive because of purple dye seen under the microscope. Microbe B was a…

    Words: 836 - Pages: 4
  • Gram Staining Experiment

    Introduction Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry.The aim of this experiment. Gram staining was the first differential technique used and still in use to make microscopic vision of bacteria clearer while also sub-divides the bacteria into two major groups: Gram-positive and Gram –negative. This experiment should help us describe how Gram staining…

    Words: 863 - Pages: 4
  • Essay On Gram Staining Procedure

    Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…

    Words: 915 - Pages: 4
  • Gram Staining Lab Report

    Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the…

    Words: 1611 - Pages: 6
  • Gram Staining Research Paper

    Gram staining is a technique where certain stains are used to identify bacteria. It was discovered in 1884 by Hans Christian Gram where he used stains to differentiate bacterial cells in diseased lung tissue. Gram staining has evolved over time to become a trusted and common diagnostic tool used in clinical and research settings. Gram stains are mainly used to identify unknown bacteria. It is often the first test used on an unknown sample of bacteria. For medicinal uses gram staining is commonly…

    Words: 1182 - Pages: 5
  • Gram Staining Lab

    Information about the Bacteria Pseudomonas aeruginosa During microbiology lab, an unknown bacteria culture in liquid broth was assigned to be identified by conducting a series of various tests. Nearly twenty different tests were conducted on the bacteria, but the most important of these was Gram staining test, gelatin stab test, and oxidase test. The results of these three tests allowed for the determination of the bacteria genus and species. The first test conducted was Gram staining. In the…

    Words: 801 - Pages: 4
  • Microscope Lab Report

    Lab Five: Microscope and Simple Staining Introduction A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a…

    Words: 855 - Pages: 4
  • Gram Catalase Test Report

    It is also called differential staining because it differentiates among bacteria and can be used to distinguish between them based on the differences of cell wall structure. There are two major groups of bacteria, the gram positive staining cells and gram negative staining cells. In this procedure, the bacteria are first stained with crystal violet “basic stain”, and then treated with adding a mordant, iodine, which fixes the stain inside the cell. The stained cells are washed with a…

    Words: 1163 - Pages: 5
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