Staining

acidic, which cause your teeth to erode. You should consider eating broccoli or kale after you eat pasta sauce. Dark green vegetables have a film that protect the teeth from staining. Ice Pops Ice pops are a great summertime treat. However, they can wreak havoc on your teeth. They can make your teeth more susceptible to staining. The cold temperatures can also damage your teeth. Tea Tea has…

Lab Five: Microscope and Simple Staining Introduction A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a…

It is also called differential staining because it differentiates among bacteria and can be used to distinguish between them based on the differences of cell wall structure. There are two major groups of bacteria, the gram positive staining cells and gram negative staining cells. In this procedure, the bacteria are first stained with crystal violet “basic stain”, and then treated with adding a mordant…

organisms on earth and the most important. Gram staining is used by scientists to help identify and classify bacteria due to the fact that it is difficult to discern unstained bacteria through light microscopes (1). Since bacteria are small organisms that are hard to distinguish through microscopes, the staining allows for the determination of shape (2). The staining is affected by the composure of the cell wall and how it can retain the dyes (4). The staining not allows for many…

Legionnaires' disease is known as a " Gram-negative bacteria". This means that during a Gram staining test the Legionella doesn't keep the pink dye. The reasoning behind this is because of the "cell wall composed of a thin layer of [peptidoglycan]" (Medicinenet.com). A counter stain is later added to make sure the bacteria being tested is surely Gram-negative. Legionnaires' disease is caused by the Legionella bacteria. In order to acquire the disease you must first obtain the Legionella…

Cresyl Violet (CV) staining protocol used in the laboratory. Cresyl Violet is a dye used to stain brain tissues and it is commonly used in histological sectioning. The dye is basic and therefore it binds to ribosomes that are RNA rich and nuclei of cells, which are acidic. Also, the dye leaves a purple color on the stained neuronal tissues. For the purpose of most of our studies, Cresyl Violet staining is used to analyze the lesion size and location in the brain of mice. This staining is…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

A. Description of the Study Area The experimentation of the study will be conducted in PLM College of Science Laboratory at Pamantasan ng Lungsod ng Maynila, Gusaling Lacson, 6th floor. The University is located beside Department of Labor and Employment building in Intramuros, Manila, known as the “Walled City”. At the back of the University is the Philippine Red Cross - Manila Chapter. The Lacson Building where the laboratory is located is in between the University Gymnasium and Justo Albert…

In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

If different abiotic environmental variables are offered to an earthworm, will they prefer one over another? We planned and carried out investigations by planning the experiments the worm was going to do, and we carried out investigations by investigating and writing down what happened. One cross-cutting concept that was in the lab was patterns because 3 out of 4 experiments ended in the worm picking the same thing 3 times. The first hypothesis is that if worms are placed in an area with light…