Staining

The preparation for this lab should be done a week prior to examining each layer. The preparation consists of compiling the pond. To do so, obtain a small, disposable bowl with a cover. Using a thumbtack, poke holes into the lid of the container. Then, using black paper, wrap it 2/3 of the way around the bowl and proceed to the securing the black paper with aluminum foil. Once that’s completed, fill the bowl 1/4 way with soil and hay. Next, the bowl is filled with water 3/4 way. Cover the bowl,…

Introduction Identifying unknown bacterium is not only vital to microbiology, but also to the medical and pharmaceutical industries in order to treat disease/infection properly. Bacteria are classified and identified to distinguish among strains and to group them by criteria of interest (1). There are several different procedures that can be utilized to identify a bacterium. This includes the oxidation test, gram stain, and catalase test. The purpose of this lab report is to show the…

viewer is aware of what type pf bacteria is being utilized. Both of these criteria in a lab are met with the use of a staining procedure which allows for greater contrast between the mostly translucent cells and light used by the microscope. Staining is a critical step when analyzing microorganisms and has great significance in a laboratory or clinical setting. The two different staining…

found that there was no bacterial growth outside of the stab line in the agar which indicates the unknown as being nonmotile. The unknown microorganisms morphology and the arrangement were identified after laboratory four through simple staining. Single staining consists of a single dye which informs only about cell shape and size, rather than differences between cell components. Through the simple stain, it was found that the unknown is a staphylococcus microorganism which is spherical and in…

cell wall structure, and other characteristic using the results from tests such as gram staining, simple staining, and spore staining. According to my lab notebook, the result from gram staining of my known is seen to be a rounded shape, cocci shape all bunched and clustered together like a bunch of grapes at 100x using the microscope (Kaddo, 2017, pg. 22). The color after the safranin wash from gram staining…

isolated and stained to find out whether it is Game positive or Gram negative, after Gram staining, there will be an ability to view the morphology or shape of this bacteria. The doctor can further test for the bacteria present, after the doctor uses gram staining, they can then use the catalase test, and polymerase chain reaction (PCR) tests to further positively identify the…

inoculated with our unknown organism it was allowed to be incubated at 35 degrees Celsius for 24 hours. After the plates had undergone incubation for the full 24 hour duration, color and morphology was observed and noted for our unknown organism. Gram staining was than conducted after, in which we determine if the unknown organisms was either positive or negative based on the thickness of its peptidoglycan wall that was able to retain the crystal violet color or not. This determination lead to…

Timing of each chemical used in the Gram staining is very important for an accurate outcome. Time for the crystal violet was changed from 20 seconds to 15 seconds to prevent over staining (Harley,…

The identification of Escherichia coli as the unknown was concluded by a string of tests and observations such as gram-staining, shape, motility, oxidation, fermentation and citrate tests. This began with gram-staining the organism. After viewing beneath a light microscope, the unknown showed pink rods, proving to be gram-negative. The observation of motility was made by viewing the bacteria moving towards a specific direction. The next tests utilized in determining the unknown was oxidation…

in this environment. This project’s purpose is to identify an unknown bacterium by various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test, Motility, Triple Sugar test, Carbohydrate Fermentation test, and the IMViC test. Especially, the Gram stain differentiates between two large groups of microorganisms. Plan…