Staining

2 have pellicle, a layering of growth, of cells at the top. In this lab, the main purpose of this experiment is to identify the biochemical characteristics of an unknown bacteria by using first the Gram Stain and based on the results of the gram staining, several biochemical tests can be performed to identify with accuracy the unknown bacteria. II. Identification Bacteria are divided into Gram Positive and Gram Negative based on the chemical composition of the cell wall. Gram stain, a…

identification and narrowing down all the possible tests to continue for identifying. Specific biochemical tests were performed. The biochemical tests performed were chosen based on the identification table that was given from the lab manual based on gram staining. The first test I performed was to check for endospore forming, my results came back positive the first time. However, it was difficult to verify if it was positive or not which had me test three more times resulting in non endospore…

The first procedure that needed to be done to identify the organism was a simple stain. Simple staining determines the micrograms morphology. The next procedure that had to be performed was a gram stain. Gram staining determines whether the microorganism is gram positive or gram negative. After doing these procedures it was determined that the organism was a gram positive cocci. The biochemical test performed…

that the bacterium was bacilli (rod) shaped. This was determined by viewing individual cells through a microscope at 400x power. Secondly, that the bacterium was most likely a Gram-stain-negative because it appeared to be a pink color after gram-staining. This had several implications: The experimenters now knew the bacteria’s cell wall composition, its outer membrane composition, peptidoglycan concentration, outer membrane amount, and more. Because of the gram stain results, it is evident that…

Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate…

cell. To begin, place a drop of methylene blue on a slide, then smear a toothpick amongst the inside of the cheek, lastly smear the toothpick onto the stained slide. Cheek cells just like starch cells are hard to be viewed without the addition of a staining chemical, in this case, the methylene blue will make the cheek cells have a slight blue tint so they are more easily observed. Place the slide on the stage of the microscope then view and center the cheek cells under the scanning-power…

The goal of this experiment was to identify as well as learn about an unknown species of bacteria along with learning some techniques when it comes to process of elimination in a scientific study. To do this, many different tests were completed to help rule out species that were provided on a sheet of paper listing the possible candidates for the unknown microbe. The Bergey’s Manual of Determinative Bacteriology flow chart was also used to narrow down choices as tests were completed, along…

Determining the cell shapes will help with the process of elimination organisms. Under microscopic examination for my unknown 16 broth, it appears to be a coccus shape and a gram positive. Gram staining can determine cell morphology, size, and arrangement. In a positive gram stain, a primary stain called crystal violet is used to stain the cell. For every new stain, mordant, decolorizing agent, and counterstain, it must be rinsed with distilled…

hospital acquired infection. Gram negative cocci Neisseria gonorrhoea needs raised carbon dioxide levels to be cultured, bacteria appears in pairs (diplococcic) Gram negative: Gram-negative bacteria are bacteria which do not turn purple in the Gram staining process used as a basic step in the identification of bacteria. Most bacteria can be divided into either Gram-positive or Gram-negative bacteria, reflecting key differences in the composition of their cell walls. These differences often…

My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified. We identified that K.…