Staining

After completing several thorough procedures to discover my unknown bacteria, bacteria #304, it was discovered to be Escherichia coli or for short E.coli. I can conclude that my answer is correct because several tests were conducted leading me to the specific bacteria. The first step taken to discover the unknown bacteria was a gram stain. A section of the unknown bacteria was taken and stained with crystal violet and safrinin so that the bacterium was visible under a microscope. The gram stain…

After the incubation period, the agar containing the antibiotic testing was taken out and if the microbe reacted with the antibiotic, a circle or clearing would appear around the disk containing the antibiotic. The diameter of this circle was then measured and compared with a table found on page 95 of the Microbiology Lab Manual to understand the antibiotics resistance, intermediate and sensitive levels, as they differ for each antibiotic. The numbers on the table in the Microbiology Lab…

“See if you can guess what I am now … I’m a zit. Get it?” - Bluto Background: The purpose of the antiseptic lab was to show the effectiveness of multiple antiseptics: hydrogen Peroxide, Purell hand sanitizer, Water and Iodine. The procedure of the lab for the first day involved our group acquiring a cotton swab and using the swab to gather the Propionibacterium acnes (P. acnes) bacteria from various spots around our face, we then grabbed a Petri dish filled only with a thin layer of agar. We…

After, then other biochemical tests were performed that were specific to the gram- negative bacteria to start the identification process of the unknown. The results of the gram negative tests lead to the identification of the bacteria Alcaligenes faecalis. The gram stain procedure was the first test performed by isolating a colony from the pure culture streak plate. The gram stain was followed orderly right from the referenced laboratory manual. The test resulted to be Gram- Negative bacteria…

Throughout the course of the semester we have learned how different variables in an environment can affect the growth, change, and behavior of an organism. Using this research as a guide we set out to design an experiment that would show a particular variable’s effect on brine shrimp eggs. After much debate we decided to use light wavelengths as the variable being tested. We wanted to see the difference in development in the brine shrimp eggs and the development after hatching when…

Abstract 6- bromo-2-(4-chlorophenyl)quinazolin-4(3H)-one derivatives 2-7 were synthesized.. The synthesized compounds were evaluated for their anti-microbial activity using the agar diffusion technique . The tested compounds were evaluated against, Gram +ve bacteria , Gram -ve bacteria, Yeast and Filamentous Fungus. Minimum inhibitory concentration (MIC) was made in vitro for compounds which showed antimicrobial activity. Compound 3b has broad spectrum activity against Gram +ve bacteria, Gram…

Mixed Unknowns This report discusses a series of tests along with results done in order to determine two bacteria in a given broth. For this experiment we were provided with an unknown broth, along with the supplies and media to perform the needed tests that would determine what bacteria the broth contained. We were also provided with a chart of possible bacteria. All tests were preformed with aseptic and sterile techniques. After being given the broth, using sterile methods I streaked a TSA…

3. I chose the plate with dilution factor 10-7 to count. This was the only plate that yielded an appropriate CFU, therefore, it was the sample with the highest countable colonies. The plate with the dilution of 10-6 yielded Too Many To Count (>300) while the plates with dilution 10-8 and 10-9 yielded Too Few To Count (<30). 4.If I were to do a total count on this sample, as opposed to a viable count, the total count would be higher because it accounts for all cells, not just visible and…

How does the location of a body part effect the bacteria population present on the said part? What has more bacteria the inside of a person's mouth or a person's index finger? My biology class is learning how to conduct experiments, and last week for our experiments we were broken into groups and given instructions to swab any two different objects. After swabbing our two objects, we were then suppose to wipe each swab onto a divided petri dish in order to see which object had more bacteria.…

Bergey’s manual was used to identify the unknown to be either from genus Flavimonas also known as Pseudomonas or Frateuria based on the results of the experiments. Multiple different experiments were conducted to identify the unknown, however only seven characteristics of the unknown were discovered from the data in Bergey’s manual. Not all test performed in lab were used due to the fact that some tests were not listed on the manual, while others did not apply to the category of the bacteria.…