Escherichia coli

present in order for the GFP to be physically active and visible. The arabinose C gene also present with the GFP recognizes and binds to arabinose so that they gene may produce the glow. A common bacteria used in this process is Escherichia coli, better known as E. coli. It is a small, single-celled bacterium with a rapid reproduction rate, making it ideal to use in transformations since the effects will be seen much faster than with other hosts. When the bacterium receives the genes from the…

E. Coli Disease Research Analysis When we eat out or consume food we often don 't consider that there may be a risk of contracting illnesses and diseases. As consumers though, we should give this consideration as it’s a very real possibility. Escherichia coli is a natural anaerobic bacteria found in the intestines of animals and humans. However, there are other forms of this bacteria that can cause infections and disease (Laupland et al., 2008).The infectious bacteria can become a food borne…

Assignment 2: Galactose Operon of E. coli FS 8404 Professor Mustapha Of some 4,000 genes in Escherichia coli (E. coli), only a handful are expressed at a time1. This regulation in gene expression helps cells function more efficiently. To provide the cell with energy, glucose is the most highly desired source. However, in the absence of glucose and the presence of other sugars, operons regulate gene expression to code for enzymes that can help utilize the sugar as energy in the cell1. Galactose…

coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis)), Nutrient agar plate Experiment 1.5 commenced by dividing the Nutrient agar plate into four equal segments. Aseptic technique was used to pick up a sample of broth culture using…

take up genetic material (in the form of naked DNA) from the environment. In this lab, the process of transformation was conducted by using a plasmid containing various genes called pGLO along with the bacterium Escherichia coli. In order for transformation to occur in Escherichia coli, repeated washes in the presence of CaCl₂ followed by heat shock treatment (42°C for 50 seconds) occurred to induce the competence of the bacterium. Successful transformation results were expressed by phenotypic…

risk that pathogens are present. If indicator organisms are present in large numbers, the contamination is considered to be recent and/or severe. Total coliforms, fecal coliforms, and Escherichia coli are indicators of water quality. When other coliform organisms are found without presence of faecal coliforms and E. coli, other indicator organisms can be used to confirm the presence of faecal contamination. The two most common indicators are faecal streptococci and the sulphite-reducing…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

gives a variety of different results that are easy to interpret. A metallic green color indicates either Escherichia coli or Citrobacter freundii, purple can indicate Enterobacter aerogenes, and a pink color occurs when Serratia marcescens is plated on an Eosin Methylene Blue plate. Upon entering the lab, I discovered metallic green colonies on the plate. I then knew I had either Escherichia coli or Citrobacter freundii. To find out which I chose to inoculate a tryptophan broth with my…

The purpose of this experiment was to determine the working concentration of Tetracycline (TNC) on Escherichia coli (EC). We used EC because of its characteristics of fast growth in different environments, complete gene sequence, and availability. Our antibiotic, TNC, is a broad spectrum antibiotic which is effective to many types of bacteria. This is because it obstructs tRNA anticodon from reading the mRNA codons in the 30S ribosomal subunit which halts the lengthening of the protein chain.…

coli for some individuals. A recent study concluded that some people carry genes in their DNA that make them immune to a certain strain of the E. coli bacteria (“Immunity genes”, 2016). This is a promising find. Building upon this information and research may create a cure for these types of bacterial infections in the future…