Escherichia coli

laboratory of Fasa University of Medical Sciences: Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 29212, S.saprophyticus ATCC 15305, Enterococcus fecalis ATCC 29212, Proteus vulgaris PTCC 1079, Klebsiella pneumoniae ATCC 10031, Escherichia coli ATCC 25922. The leaves of C. viminalis were collected from the decorated trees in the city of Fasa in April 2015. The plant identity was confirmed in Department of Botany, Darab Payame Noor University, Iran. After cleaning, leaves…

The field of medicine although obtained remarkable progress in recent decades, infectious illnesses triggered by pathogenic harmful bacteria are still an important risk to public wellness globally (1). Great attention has been paid to the anti-microbial level of resistance due to deaths and death rate from illnesses triggered by proof bacteria(2). Klebsiella pneumoniae is a Gram-negative bacteria that can cause different kinds of medical care associated attacks, such as pneumonia, bladder…

possibility because of techniques like transformation of genetically engineered bacteria. Although, manipulating bacterial gene expression has many great implications, it can also be quite harmful. By manipulating gene expression of bacteria, such as Escherichia coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was conducted in order to transform E.coli into an antibiotic-resistant bacteria by…

Experimental trials were performed by students over a timeframe of three weeks to determine the identity of three unknown bacteria contained in broth medium stored in labeled test tubes that were randomly assigned containing: one Gram positive bacterium, one Gram-negative paracolon, and one Gram-negative coliform (Carson, 2016). The unknown sample assigned in this experiment was #19. After initially streaking the solution on clean streak plates and TSA agar, the identity was determined from a…

How often do you get thirty? Does a glass of water seem to do the job? The research question I am investigating is What is the preferred type of drinking water, NYC bottled water of tap water? This research question is important because we need to know whether the best tasting water is the one most people drink. This research shows the repeatability of a procedure in a different setting because this research has been done before but not with the water types I’m using and my location. In this…

Biochemical Unknown I. Introduction Cultural Characteristics or morphology and biochemical tests can be used to identify and classify microorganism. By culturing microorganism in nutrient broth, slants, and on nutrient agar plates, the cultural characteristics or morphology can be determined. In this lab, the test tube 2 was incubated in a nutrient broth. The pigmentation of the tube was yellow. The media for test tube 2 was turbid. There were a few sediment on the bottom of the broth. In…

A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…

The gas generation is due to E. coli metabolic processes that generates acid and changing the turbidity. 2. The Membrane Filtration Method did work as well. MF method can replace the MNP test for microbiological analysis of water sample. After the incubation of the mEndo plate for 24 hours it is perceptible the grown of E. coli colonies across the plate. The results of this method are shown in the figure 4. As it was used mEndo, just the E. coli colonies form bright green colonies with…

It is important to make an accurate diagnosis in this case as the risk of severe illness and treatment varies between an uncomplicated and a complicated UTI.1 The absent of classical presentation of loin pain and rigors would make acute pyelonephritis very unlikely. She was also haemodynamically stable on examination and showed no signs of urosepsis. Hence, based on this patient’s complaint of dysuria, frequency and suprapubic pain accompanied by urinalysis findings of leucocytes and nitrites;…

Genetic transformation is the process in which a gene from one organism inserts itself into a different organism thus changing that organism’s genetic makeup, “Genetic transformation literally means change caused by genes and involves the insertion of a gene into an organism in order to change the organism’s trait.” Abraham, Anup.2016. Bacterial Transformation. Biology 281 Conceptual Approach to Biology for Majors I. Arizona State University. Hayden-McNeil. In this experiment, bacteria will be…