Human Genetic Transformation Experiment

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Genetic transformation is the process in which a gene from one organism inserts itself into a different organism thus changing that organism’s genetic makeup, “Genetic transformation literally means change caused by genes and involves the insertion of a gene into an organism in order to change the organism’s trait.” Abraham, Anup.2016. Bacterial Transformation. Biology 281 Conceptual Approach to Biology for Majors I. Arizona State University. Hayden-McNeil. In this experiment, bacteria will be used in an attempt to perform genetic transformation, “Naturally competent bacteria are able to take up exogenous DNA and undergo genetic transformation.” Dubnau, David. 2004. DNA uptake during bacterial transformation. Nature Reviews Microbiology. 2, …show more content…
Figure 1 consists of the predictions that were made for this experiment. These predictions were based off of what was known about genetic transformation and the variables used to conduct the experiment.

Figure 2: Observation of Plate Outcomes

more than 300 colonies: +++ LB: Luria broth is a food and nutrient source for E. coli.
5-300 colonies: ++ Amp: Ampicillin is an antibiotic that will kill E. coli.
1-4 colonies: + Ara: Arabinose is a monosaccharide no colonies: - -pGLO: indicates the E.coli were never exposed to pGLO plasmid +pGLO: indicates E.coli were exposed to pGLO plasmid.

Figure 2 consists of the observations made from the experiment that was conducted. These observations were made after viewing the five plates under a UV Light.

Image of Plates Under UV Light

The image above depicts the five plates tested during the experiment under a UV
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All of the plates in this experiment that contained an arabinose sugar experienced relative growth and were fluorescent under a UV light. Furthermore, the initial predictions made were similar to the observed outcomes for this experiment. Whether a bacteria would glow under the presence of a UV light was predicted probably as well as whether or not it would experience relative growth. However, the relative growth predicted in terms of colony size was not consistently supported by the results. A source for potential error in the experiment is my lab group left the tubes in the ice bath for around four minutes rather than three minutes. Additionally, the transfer of the tubes from the heat bath back to the ice could have been performed more efficiently. These errors could have possible affected the results of the experiment. A similar experiment conducted by Michael G. Lorenz and Wilfried Wackernagel of Oldenburg university suggests that transformation of genes in bacteria is a natural process, “The results of in vitro studies on the transformation of many bacterial species and strains, the evidence for the existence of extracellular DNA in the environment, the demonstration for the ability of that DNA to uptake in bacteria, and the experiments showing that bacteria can develop under the

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