Genetic Transformation Lab Report

Genetic transformation is the process in which a gene from one organism inserts itself into a different organism thus changing that organism’s genetic makeup, “Genetic transformation literally means change caused by genes and involves the insertion of a gene into an organism in order to change the organism’s trait.” Abraham, Anup.2016. Bacterial Transformation. Biology 281 Conceptual Approach to Biology for Majors I. Arizona State University. Hayden-McNeil. In this experiment, bacteria will be used in an attempt to perform genetic transformation, “Naturally competent bacteria are able to take up exogenous DNA and undergo genetic transformation.” Dubnau, David. 2004. DNA uptake during bacterial transformation. Nature Reviews Microbiology. 2, …show more content…
The bla gene codes for beta lactamase which promotes antibiotic resistance for drugs classified as being a part of the penicillin family. In the pGLO plasmid there is a unique gene regulation system which controls the transcription of the green fluorescent protein. In order for the GFP to be expressed, arabinose sugar must be present. Arabinose sugar reacts with the AraC protein, and this reaction initiates transcription of the GFP gene. The purpose of this experiment is to see whether bacteria will be successfully transformed to express the green fluorescent protein. The hypothesis for this experiment is that in the presence of an arabinose sugar, a GFP protein will be produced, and as a result bacterial transformation will occur. Genetic transformation plays an important and beneficial role in our society. For example, in agriculture genetic transformation can result in genetic traits that prevent plants from being affected by factors such as frost and pests. Additionally, genetic transformation has a significant role in medicine. Gene therapy is now being used to treat diseases that are a result of genes functioning

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