Coliform bacteria

Foodborne illness is probably the most common public health problem in the United States and around the world. It is estimated that millions and millions of Americans gets sick by consuming contaminated food or water each year. Hence it is important to detect the presence of disease-causing bacteria in drinking water. One the simplest way to tell if there is a disease-causing pathogen in the water is the presence of coliform. Just like most of the microorganisms, coliform bacteria, a Gram-negative bacteria that belongs to the bacterial group Enterobacteriaceae, can be found mostly in soil, surface and water. In addition, coliform can be found in the digestive tract, especially in the intestines of animals and humans. While coliform bacteria…

CEE 467 – ENVIRONMENTAL MICROBIOLOGY NAME: ERICK PEREIRA DANTAS DATE: SEPTEMBER 4th, 2014 PROFESSOR: DR. MORTEZA ABBASZADEGAN TA: LAUREN MCBURNETT LABORATORY #2 – BACTERIOLOGICAL EXAMINATION OF WATER: MOST PROBABLE NUMBER TEST AND MEMBRANE FILTRATION METHOD ABSTRACT: The most probable number (MPN) test and the membrane filter (MF) method have as goal to detect and estimate the presence of coliforms in a sample. Their importance in the field of environmental engineering is to support…

organisms (such as bacteria, viruses, and protozoa) into a water source (surface or groundwater). As water (surface runoff) resulting from the storm or precipitation or snowmelt runs over the land surface, it will pick up potential pollutants include pathogenic organisms as it runs over livestock excrement from barnyards, pastures, rangelands, feedlots. Furthermore, the discharge from the areas of land application of sewage sludge deposit or effluents discharge from wastewater into freshwater…

objective of this lab was to isolate and identify an unknown Gram positive and Gram negative bacteria through the use of various microbiological techniques. The identification of the genus and species for the unknown culture should be obtained based on the characteristics for Gram positive and Gram negative bacteria. Bacteria are prokaryotic microorganisms that lack a nucleus and therefore all its genetic information is stored in the cytoplasm of the cell. The bacterial cell envelope protects…

excreted in human faeces. The presence of these indicator organisms in water is evidence of faecal contamination and therefore, of a risk that pathogens are present. If indicator organisms are present in large numbers, the contamination is considered to be recent and/or severe. Total coliforms, fecal coliforms, and Escherichia coli are indicators of water quality. When other coliform organisms are found without presence of faecal coliforms and E. coli, other indicator organisms can be used to…

• Bacteria, are single-cell microorganism with no nucleus that had a sphere, rod, and spiral shape and branching threads. Ranges in diameter between (0.5- 1) µm and in length between (2-4) µm however, they are either a parasite or live independently they are found in all living things and in all environments. Some bacteria in drinking water can cause disease such as salmonella, total coliform (including fecal coliform and E.coli), Shigella, Legionella, and Campylobacter and “their presence…

viruses in groundwater. Waterborne outbreaks often occur, and are probably caused by viruses in drinking water. Because viruses are hard to detect in humans and water post-outbreak, most outbreaks are unrecognized and underestimated. Waterborne virus outbreaks in groundwater systems tend to be uncommon, so some researchers speculate that natural waters could inactivate the virus, or that some viruses need a higher concentration to be infectious. These viruses remain dormant, yet infectious, in…

Introduction: The purpose of the tests (culture media, motility, enzymes, etc.) we preform on the numerous bacteria in lab is to show any biological or chemical characteristics of the bacteria that may help it survive or adapt in the wild, how it may be useful or hazardous to humans, and use as general knowledge to differentiate bacteria from each other. In the Culture Media test we can use solid and liquid media to grow multiple bacteria in lab. It can help us determine what processes the…

variety of settings; from clinical diagnostics to environmental monitoring (1). Species of bacteria can differ fundamentally in aspects ranging from morphology to metabolism. Exploiting these differences can allow for identification of unknown organisms. Gross morphological characterizations are crude, and better replaced by more accurate and specific tests. At a fundamental level, tests such as the Gram stain divide bacteria in to two broad categories based on the characteristics of their cell…

From the patient, unknown bacteria #618 was identified to be Escherichia coli. It is apart of the Enterobacteriae genus that according to Brown’s Microbiological Applications1 are gram-negative bacteria that usually live inside the intestines of animals and humans. The organism can also live in the vagina and urethra. The data collected after isolating the organism and executing a series of tests have confirmed previous observations of E. coli in past scientific history. For the majority of…