Filtration

at the Boston Production Facility (BPF), a viral clearance filtration step must be introduced into the manufacturing process. The proposed viral reduction step would be added after the last purification column step and prior to the final formulation of the drug substance. A laboratory scale study was completed and concluded that the addition of a viral clearance filtration step to the Unclogerall® manufacturing process reduces the viral risk level to align with FDA requirements for Biotechnology products derived from cell lines of human or animal origin. This memo will describe the new process step and will evaluate the…

CEE 467 – ENVIRONMENTAL MICROBIOLOGY NAME: ERICK PEREIRA DANTAS DATE: SEPTEMBER 4th, 2014 PROFESSOR: DR. MORTEZA ABBASZADEGAN TA: LAUREN MCBURNETT LABORATORY #2 – BACTERIOLOGICAL EXAMINATION OF WATER: MOST PROBABLE NUMBER TEST AND MEMBRANE FILTRATION METHOD ABSTRACT: The most probable number (MPN) test and the membrane filter (MF) method have as goal to detect and estimate the presence of coliforms in a sample. Their importance in the field of environmental engineering is to support…

Abstract: Using the PhysioEx.9.1 software, I ran Activity 4: Simulating Filtration lab to learn about the filtration of solutes in a membrane system. This experiment was conducted using four solutes: NaCl, urea, glucose, and powdered charcoal. The goal was to learn if any of these solutes passed through the three different dialysis membranes at varying pressures. Does increasing the pore size increase filtration rate? What effect will pressure have on the filtration rate? These are the…

Bio-filtration Bio-filter is considered the most important and efficient separation process that is used in the elimination of organic pollutants from water sources. The article focus on the biological processes that are used in bio-filtration and determination of their effectiveness (Acero, 2009). Full-scale laboratory applications of wastewater treatment and bio-filter are discussed and compared with their pollutant removal ability. Filtration is defined as necessary treatment process used…

Introduction Filtration is a passive process in which solutes pass through a membrane from high pressure to low pressure. The amounts of solutes that pass through the membrane are dependent upon the amount of pressure and the size of the membranous pores. Solutes such as blood cells and proteins are too large to pass through so capillaries retain them. An example of filtration in the body is renal filtration, a process in which the blood is filtered in the kidney so that substances that are…

The British rulers required different kinds of workers to maintain the business and the administration. 2. The legislature did not have adequate assets for teaching the majority. 3. The informed individuals instructed on British lines through English medium would get higher posts in taxpayer driven organizations and after that normally they would utilize their impact in controlling the majority from conflicting with the administration run the show. 4. Higher classes taught through the…

Glomerular filtration is the physical method the kidney uses to sort what should be excreted from what should be kept in the bloodstream. In the first part of the process, a membrane called the filtrate membrane filters out red blood cells, large proteins, and other large plasma solutes. For example, albumin is filtered out at this point, with only a tiny amount remaining in the subsequent glomerular filtrate. Because the afferent arteriole to the glomerulus is much larger than its efferent…

Gel beads are present on a column and, as the mixture (in this case liposomes) flows through the column, aspects of the mixture can be trapped via binding to, or entering, the gel beads. This occurs on a size related basis, with molecules of specific size being entrapped by the column (Ruysschaert et al., 2005). In the case of liposomes, their large size causes them to be entrapped between the gel beads (lots of gel must be present to stall the liposomes for a more hydrophobic environment) while…

How does light filtration affect the rate of photosynthesis? Introduction: Photosynthesis is an essential process plants use to convert light energy from the sun into chemical energy, released as glucose. Some of the limiting factors of photosynthesis include temperature, amount of light, the concentration of carbon dioxide, availability of water and the concentration of chlorophyll. Fig.1- The word and symbol equations for photosynthesis. The aim of the practical is to model the rate of…

Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…