Introduction Filtration is a passive process in which solutes pass through a membrane from high pressure to low pressure. The amounts of solutes that pass through the membrane are dependent upon the amount of pressure and the size of the membranous pores. Solutes such as blood cells and proteins are too large to pass through so capillaries retain them. An example of filtration in the body is renal filtration, a process in which the blood is filtered in the kidney so that substances that are essential can be retained and reabsorbed by the body( biology online). The objectives of this experiment are as followed:
1. To understand that filtration is a passive process depending on a pressure gradient.
2. To understand that filtration is not a selective process.
3. …show more content…
To explain that the size of the membrane pores will determine what passes through.
4. To explain the effect that increasing the hydrostatic pressure has on the filtration rate and how this correlates to events in the body.
5. To understand the relationships between molecular weight and molecular size. (physio ex)
Materials and Methods
Materials
• Top and bottom beakers-used for filtration of solutes
• Dialysis membranes with various molecular weight cutoffs (MWCOs)
• Membrane residue analysis station-used to analyze the filtration membrane. (physio ex)
Methods
1. Drag the 20 MWCO membrane to the membrane holder between the beakers.
2. Increase the concentration of all solutes to 5.00 mg/ml, dispense and start.
3. Drag the membrane to the analysis unit and begin the analysis.
4. Record your data then flush the beakers to prepare for the next run.
5. Drag the 50 MWCO membrane to the membrane holder between the beakers.
6. Repeat steps 2-4.
7. Drag the 200 MWCO membrane to the membrane holder between the beakers.
8. Repeat steps 2-4.
9. Increase the pressure above the beakers to 100 mm Hg.
10. Repeat step 7.
11. Repeat steps 2-4.
(physio ex)
Results
Table 1- Results of
1. To understand that filtration is a passive process depending on a pressure gradient.
2. To understand that filtration is not a selective process.
3. …show more content…
To explain that the size of the membrane pores will determine what passes through.
4. To explain the effect that increasing the hydrostatic pressure has on the filtration rate and how this correlates to events in the body.
5. To understand the relationships between molecular weight and molecular size. (physio ex)
Materials and Methods
Materials
• Top and bottom beakers-used for filtration of solutes
• Dialysis membranes with various molecular weight cutoffs (MWCOs)
• Membrane residue analysis station-used to analyze the filtration membrane. (physio ex)
Methods
1. Drag the 20 MWCO membrane to the membrane holder between the beakers.
2. Increase the concentration of all solutes to 5.00 mg/ml, dispense and start.
3. Drag the membrane to the analysis unit and begin the analysis.
4. Record your data then flush the beakers to prepare for the next run.
5. Drag the 50 MWCO membrane to the membrane holder between the beakers.
6. Repeat steps 2-4.
7. Drag the 200 MWCO membrane to the membrane holder between the beakers.
8. Repeat steps 2-4.
9. Increase the pressure above the beakers to 100 mm Hg.
10. Repeat step 7.
11. Repeat steps 2-4.
(physio ex)
Results
Table 1- Results of