Chromatography

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  • Essay On Liquid Chromatography

    impact the environment, including water treatment processes. The purpose of these experiments is to identify the compounds in aquatic samples from various places throughout the world. Verifying these compounds is crucial in determining how drugs of abuse effect the environment. Ultraperformance Liquid Chromatography- Tandem Mass Spectrophotometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters1 Ultraperformance liquid chromatography is the best method for analyzing drugs of abuse in different substances including wastewater. Liquid chromatography reduces analysis time and increases efficiency. The purpose of this research, Ultra Ultraperformance Liquid Chromatography- Tandem Mass Spectrophotometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters, is to develop a method for Ultraperformance Liquid Chromatography of various synthetic drugs along with their metabolites. The newly established method was later related to estimation occurrences…

    Words: 1473 - Pages: 6
  • Gas Chromatography Essay

    Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…

    Words: 940 - Pages: 4
  • Gas Chromatography Lab

    Introduction: The main purpose of this experiment was to take the Gas Chromatography/ Mass Spectrum part of the “Analysis of US bills for cocaine using gas chromatography / mass spectrometry” lab and improve upon the gas chromatographic results. This would be done by finding a way to separate the peaks of cocaine and doxepin. The lab done previously showed the use of two different methods to obtain samples of cocaine and doxepin for spiked dollar bills. The two extraction methods were…

    Words: 1038 - Pages: 5
  • Lab Analysis: Reverse Phase Chromatography

    HPLC column can be categorized into two types • Reverse Phase • Normal Phase 3.4.1.1. Reverse Phase Chromatography Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species. 3.4.1.2. Normal phase chromatography Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.[38]. A detection…

    Words: 715 - Pages: 3
  • Column Chromatography Lab

    Methods Column chromatography was set up using a 60 ml syringe clamped to a ring stand. The syringe was plugged using a small amount of cotton. An Erlenmeyer flask was placed underneath the column to collect any excess material. Eleven 20 ml vials were labeled numbers 1 through 11. Silica gel was measured to 42 ml using a graduated cylinder and then moved into a 150 ml beaker. A slurry was created by adding 50 ml of hexane to the beaker containing the silica gel. The slurry was stirred for…

    Words: 877 - Pages: 4
  • High Performance Liquid Chromatography

    Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

    Words: 1867 - Pages: 8
  • Paper Chromatography Lab Report

    Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

    Words: 860 - Pages: 4
  • Affinity Chromatography Lab Report

    Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

    Words: 2097 - Pages: 9
  • Tlc And Column Chromatography

    Introduction Separation, a process by which the distinguishing between substances through physical or chemical means in a mixture may be carried out. A widely employed technique for this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be…

    Words: 1030 - Pages: 4
  • Paper Chromatography Essay

    environmental testing, they look for PCB’s in oil, and pesticides in ground water. In the food industry, they can test additives to determine concentrations of preservatives, vitamins, proteins, and amino acids.2 Chromatography is the process of separating a mixture, and has many different applications throughout the world. There are a variety of ways to conduct chromatography including column chromatography, paper chromatography, and thin layer chromatography. The particular experiment…

    Words: 825 - Pages: 4
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