Chromatography

impact the environment, including water treatment processes. The purpose of these experiments is to identify the compounds in aquatic samples from various places throughout the world. Verifying these compounds is crucial in determining how drugs of abuse effect the environment. Ultraperformance Liquid Chromatography- Tandem Mass Spectrophotometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters1 Ultraperformance liquid chromatography is the best method for analyzing drugs of abuse in different substances including wastewater. Liquid chromatography reduces analysis time and increases efficiency. The purpose of this research, Ultra Ultraperformance Liquid Chromatography- Tandem Mass Spectrophotometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters, is to develop a method for Ultraperformance Liquid Chromatography of various synthetic drugs along with their metabolites. The newly established method was later related to estimation occurrences…

Introduction: The main purpose of this experiment was to take the Gas Chromatography/ Mass Spectrum part of the “Analysis of US bills for cocaine using gas chromatography / mass spectrometry” lab and improve upon the gas chromatographic results. This would be done by finding a way to separate the peaks of cocaine and doxepin. The lab done previously showed the use of two different methods to obtain samples of cocaine and doxepin for spiked dollar bills. The two extraction methods were…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

Gas chromatography (like any other chromatography) is a separation technique in which the mobile phase is a gas. This technique is applied for the separation of volatile substances. This method separates a mixture into its components and is used to measure the concentration as well as it identifies the components. GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated. Each peak in…

Chromatography is a separation method used to help identify mixtures. One common form of chromatography is gas chromatography. This type of chromatography will usually use a gas as the mobile phase, and a liquid as the stationary phase (“Gas Chromatography”). A gas chromatograph is comprised of some vital components, which include: the carrier gas, flow controller, injector port, column, column oven, detector, and recorder (“Gas Chromatography”). The purpose of this lab was for an unknown…

Chromatography Abstract: The purpose of this lab is to use chromatography to separate and purify organic compounds. There are many different types of chromatography but most depend on the principle of adsorption. The two important components of chromatography are the adsorbent and the eluent. Adsorbents are usually solid materials that will attract and adsorb the materials to be separated. The eluent is the solvent, which carries the materials to be separated through the adsorbent. After being…

Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

HPLC column can be categorized into two types • Reverse Phase • Normal Phase 3.4.1.1. Reverse Phase Chromatography Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species. 3.4.1.2. Normal phase chromatography Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.[38]. A detection…

Column Chromatography is considered one of the main techniques of separation in addition to SDS-PAGE and gel electrophoresis. This method can be useful in both a small scale as in a laboratory experiment as well as in a larger scale at the industrial level. It is also applied in different fields ranging from biology and biochemistry to microbiology and medicine. Column chromatography is also referred to as gel filtration or size exclusion chromatography among many other terminologies and it is…