Bradford protein assay

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  • Light Spectroscopy Lab Report

    when a material is subjected to radiation, allow for the identification and analysis of unknown concentrations of compounds.1 With that being said, the purpose of this experiment is to spectroscopically assess the concentration of varying DNA and protein solutions. In order to do this, a series dilution will be prepared from bovine serum albumin (BSA) and distilled water. Next, a spectrophotometer, an apparatus that measures the intensity…

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  • Bovine Hydrochloric Acid Solution

    concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In order to prepare each of these standards within a range of 50 microliters to 1200 microliters and using a provided stock solution of 1.46 mg/mL of the protein bovine gamma globulin (IgG) different calculations were performed in order to find out the exact…

    Words: 1188 - Pages: 5
  • Affinity Chromatography Lab Report

    forms of trypsin during storage (Sugumaran). In this experiment, affinity chromatography was used to extrapolate the active form of trypsin from the inactive form of trypsin. A column containing P-amino-bezaidine was used to form a bond with the active form of trypsin in the matrix (Sugumaran). Two assays were performed. The first assay was to determine if there was any active trypsin by employing Benyzol-DL-arginie-p-nitroanilide (BAPNA), which forms an observable yellow color (from…

    Words: 2097 - Pages: 9
  • Protein Assay Lab Report

    Introduction Determining the concentrations of proteins is necessary for a variety of different reasons. For example, protein concentration must be known in order for proteins to be used in enzyme reactions, as the correct stoichiometry must be known. Another example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different…

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  • Trypsinization Of Transfer Cells

    The assay was performed as described in section 3.2.2.2. P4 concentrations were analysed in spent culture media for cells cultured for 48, 96 and 144 hr in the present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1)…

    Words: 984 - Pages: 4
  • Story Of The Yorkshire Ripper Analysis

    His first conviction was because he attempted to attack victim 36-year-old Anna Rogulskyj with a hammer and slashing her with a knife. The victim managed to escape because people heard her screams and Peter panicked, throwing him off guard and causing him to flee. When Peter heard about his mother’s death, he had a “cooling off period”. It lasted about 11 months and then he went on the spree again. He had been arrested many times by the police but they let him go because they did not have…

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  • Case Study: The Yorkshire Ripper

    Peter William Sutcliffe otherwise known as ‘The Yorkshire Ripper ' was arrested on January 1st, 1981 after a murderous spree which spanned five years and claimed the lives of 13 women and attempting to murder a further seven (see appendix for details of victims). The case engulfed the nation as one of the most brutal in modern history since that of ‘Jack the Ripper ' in the 1800 's hence why the media adopted the term ‘Yorkshire ripper ' when addressing the case. Although known for the heinous…

    Words: 1396 - Pages: 6
  • Canine Pancreatic Lipase Lab Report

    Skill #28 Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient.…

    Words: 986 - Pages: 4
  • Lateral Flow Immunoassay Article Analysis

    Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6. This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing…

    Words: 1012 - Pages: 5
  • Lactate Dehydrogenase Lab Report

    NADH is at 340nm, but the peak absorbance of NAD+ is 259nm (Powers, et al., 2007). Enzyme assays are performed to measure the rate of an enzyme-catalysed reaction, known as the enzyme activity. Many factors can affect the enzyme activity (including temperature, pH, substrate concentration), so all conditions apart from the one being quantified should be standardised. The…

    Words: 1627 - Pages: 7
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