Bradford protein assay

Homogenates of liver were provided. The homogenates were made by homogenizing liver in a 1:20 w/v (1g: 20 ml) ratio in a sucrose-phosphate buffer, in which 250mM of sucrose and 50mM NaPO, with a pH of 7.4 were added. This information was used to conduct an experiment to determine the concentration of DNA, protein, and carbohydrates in different homogenate samples, including heart, liver, and kidney of Bos taurus, while also looking for the diverse protein sizes found in each homogenate sample. The liver homogenate was then stored at -70°C. Of 300 µl, 5 µl of homogenate was placed into 2 µl of protein gel sample buffer. The homogenate was then mixed and heated at 80°C for 10 minutes. After heating, the sample was placed in a microcentrifuge…

when a material is subjected to radiation, allow for the identification and analysis of unknown concentrations of compounds.1 With that being said, the purpose of this experiment is to spectroscopically assess the concentration of varying DNA and protein solutions. In order to do this, a series dilution will be prepared from bovine serum albumin (BSA) and distilled water. Next, a spectrophotometer, an apparatus that measures the intensity…

concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In order to prepare each of these standards within a range of 50 microliters to 1200 microliters and using a provided stock solution of 1.46 mg/mL of the protein bovine gamma globulin (IgG) different calculations were performed in order to find out the exact…

forms of trypsin during storage (Sugumaran). In this experiment, affinity chromatography was used to extrapolate the active form of trypsin from the inactive form of trypsin. A column containing P-amino-bezaidine was used to form a bond with the active form of trypsin in the matrix (Sugumaran). Two assays were performed. The first assay was to determine if there was any active trypsin by employing Benyzol-DL-arginie-p-nitroanilide (BAPNA), which forms an observable yellow color (from…

Introduction Determining the concentrations of proteins is necessary for a variety of different reasons. For example, protein concentration must be known in order for proteins to be used in enzyme reactions, as the correct stoichiometry must be known. Another example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different…

The assay was performed as described in section 3.2.2.2. P4 concentrations were analysed in spent culture media for cells cultured for 48, 96 and 144 hr in the present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1)…

Skill #28 Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient.…

His first conviction was because he attempted to attack victim 36-year-old Anna Rogulskyj with a hammer and slashing her with a knife. The victim managed to escape because people heard her screams and Peter panicked, throwing him off guard and causing him to flee. When Peter heard about his mother’s death, he had a “cooling off period”. It lasted about 11 months and then he went on the spree again. He had been arrested many times by the police but they let him go because they did not have…

Peter William Sutcliffe otherwise known as ‘The Yorkshire Ripper ' was arrested on January 1st, 1981 after a murderous spree which spanned five years and claimed the lives of 13 women and attempting to murder a further seven (see appendix for details of victims). The case engulfed the nation as one of the most brutal in modern history since that of ‘Jack the Ripper ' in the 1800 's hence why the media adopted the term ‘Yorkshire ripper ' when addressing the case. Although known for the heinous…

Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6. This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime the widely used Widal testing method has a poor sensitivity as well as up to three days of delayed response time. In addition, the Widal testing…