Chromatography

Isolation of Chlorophyll and Beta-Carotene from Plant Leaves Yokoyama Yuri B1 (A0142349E) 1. Aim: a) To separate beta-carotene and chlorophyll via column chromatography. b) To analyse the contents of the plant extract as well as the separated beta-carotene and chlorophyll via thin layer chromatography (TLC) c) To analyse the absorption peaks of both beta-carotene and chlorophyll using UV-Vis spectroscopy 2. Results: Fig 1. UV-Vis spectroscopy results for S2 (Beta-Carotene extract) Wavelength at…

The liquid chromatography was performed using an Acquity UPLC® system (Waters Corp., Milford, MA, USA) coupled to a mass spectrometer (Xevo TQ-S, Waters Corp.). The bioanalytical method for the simultaneous quantitation of the three PFCs was optimized through the various…

alleged sticky bandit. The note was sent to the Tempe CSI Crime Lab for further analysis. Blaine Benson, head of the Tempe Crime Lab, has reported that paper chromatography will be used to identify the pen the bandit used. Pens from local syrup competitors were collected as evidence to compare to the ink used in the note. Paper chromatography is a common practice used to separate the colored components in the ink. The Rf value or the retention factor is the ratio of the distance traveled by the…

The genus Brassica is one of 51 genera in the tribe Brassiceae belonging to thecrucifer family, and is the economically most important genus within this tribe,containing 37 different species. Many crop species areincluded in the Brassica genus, which provide edible roots, leaves, stems, buds,flowers and seedCrops.Crops belonging to this genus are occasionally called Cole crops—derived from the Latin caulis, denoting the stem or branch of a plant.[1] Members of Brassica commonly used for food…

Several methods have been described in the literature for the determination of MNT in its pharmaceutical dosage forms or in biological samples. Examples of these methods in biological samples are gas chromatography – mass spectrometry (GC-MS) [18] in plasma and urine of rats and human while GC-FID [19] was used for MNT analysis in human urine. Also, MNT in mixture with other drugs have been assayed in human plasma using GC-FID [20]. In pharmaceutical preparations…

Calculations for Dissolved Gas Analysis in Water Samples Using a GC Headspace Equilibration Technique, EPA National Risk Management Research Laboratory, RSKSOP-175, Rev.2, May 2004. 1.2 Light Hydrocarbons in Aqueous Samples via Headspace and Gas Chromatography with Flame Ionization Detection (GC/FID), Pennsylvania Department of Environmental Protection Bureau of Laboratories, PA-DEP 3686, Rev.0, April 2012. 1.3 Lange’s Handbook of Chemistry, 14th edition (1992), McGraw Hill. 1.4 Gas…

Data/Observations: In the chromatogram of a mixture of cyclohexanol and heptanal, there are 8 peaks. Retention times are 1.783, 2.111, 2.181, 2.832, 3.798, 4.012, 4.268, and 4.351 (min) in an increasing order. Areas are 5193372, 27743, 1333, 1283, 4005, 1734332, 3526677, and 2079050. Each area corresponds to each retention times. Calculations & Results: -Student mixture (cyclohexanol and heptanal)- Retention times of peak# 6, 7, and 8 from the student mixture look similar to the standard…

Lab 1 – Ion Exchange Chromatography Danielle Small John Wright 20/01/2016 Introduction: This lab will use ion exchange chromatography – a process in which differently charged ions are separated, by altering the pH of the solution; which shifts the equilibrium of the molecules. During this lab we will separate two compounds from one solute that will be formed by amalgamating a ‘slurry’ – provided by the instructor. Resin or cellulose is used to create a sound support unit in order maintain a…

瀀瀀ract The purpose of Isolation of Bixin from Annatto Seeds using Column Chromatography was to separate and purify macroscopic amounts of pure bixin compound from annatto seed ex-tract.1 A mixture of bixin, methylbixin, and norbixin were extracted from annatto seeds. Bixin was then isolated from the mixture through the use of the column chromatography technique. The isolated bixin was characterized through Thin Layer Chromatography (TLC) analysis, which displayed an Rf value of 0.317, which…

Utilizing column chromatography and recrystallization, separation and purification of the two products was obtained. The mass of 4-NBB, the para product, was 1.57 grams, while the mass of 2-NBB, the ortho product, was 0.80 grams. The para product was expected to have the higher yield, since it is more stable and consequently harder to break down throughout the experiment. Keywords Nitration, Bromobenzene, Substitution, 4-nitrobromobenzene, 2-nitrobromobenzene, Column Chromatography, Infrared…