Buffer solution

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  • Strong Titration

    results that greatly differ then a strong-strong titration. The strong-weak titrations allow for an in depth analysis of the half-equivalence point as well as buffers. The half equivalence point allows the calculation of pH from pKa, as they are both equal at the half equivalence point, proven by the Henderson Hasslbach equation. It was Henderson in 1908 that created an equation allowing for the logarithmic calculation for pH from a buffer and the pKa which consequently lead to the knowledge and applications of buffers that we have today. Buffers were also analyzed in the experiment as buffers consist of equal molar rations of a weak acid and its conjugate base, which then…

    Words: 2238 - Pages: 9
  • Acetic Acid Synthesis Lab Report

    Acids and Bases Using the Acetic Acid and Sodium Acetate Buffer System Purpose The purpose of this experiment was to explore some of the properties of the acetic acid and sodium acetate buffer system, such as the greatest buffering capacity and the acid and base neutralization ability of several different concentrations of this buffer. Reagents The reagents used were the following: 0.10 M acetic acid, 0.010 M acetic acid, 0.60 M acetic acid, 0.60 M sodium acetate, 0.10 M sodium acetate,…

    Words: 824 - Pages: 4
  • Beta Amylase Lab Report

    Like the sodium phosphate buffer, the solid was dissolved in 300 milliliters of distilled in a 500-mL beaker using a stirrer and a magnetic stirring bar. The pH of the solution was then checked using the pH meter and it was brought down to the desired pH of 4.8 using a couple of drops of glacial acetic acid. Then it was poured into a 500-mL graduated cylinder and brought up to a volume of 1000 milliliters using distilled water. The buffer was poured into a labeled glass bottle and stored in the…

    Words: 2493 - Pages: 10
  • Le Chatelier's Principles Of The Generic Buffer System

    Buffer Systems As humans, we constantly need to regulate our bodily pH. This regulation process is called homeostasis, and is controlled by the hypothalamus and pituitary gland by a buffering system. A buffer solution is used to maintain the pH at a safe acidity. “A buffer solution is an aqueous solution consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid” (Boundless, 2010) Equation 1 – Generic Buffer Equation HA (aq) + H2O (l) --> H3O+(aq)…

    Words: 1020 - Pages: 5
  • Lab Report Catalase

    For this catalase experiment it was decided that the group was to keep the concentration of catalase and hydrogen peroxide the same and only change the level of pH buffer throughout the experiments conducted. This was done to observe the effect different levels of pH buffers have on the breakdown of hydrogen peroxide and the volume of oxygen released. The results from the majority of experiments conducted reflect that though they are in consensus with the hypothesis that the volume of oxygen…

    Words: 786 - Pages: 4
  • Essay On Effect Of Ph On Catalase

    the raw data values of the control test tubes as it demonstrated that the hydrogen peroxide and the pH buffer solution did not affect the boiling chips (which did not contain the enzyme catalase) and it was from the presence of catalase within the potato discs which caused the resulting froth…

    Words: 1472 - Pages: 6
  • Analysis Of Enzyme Lab Report

    phosphate • Pipette tips • Buffered solutions of various pH • Parafilm • Distilled water • Hot plate • Beakers • Timers • Thermometers • Incubators – water • Disposable 1ml transfer pipettes Procedure Part I: Rate of Reaction • Preparing the Spectrophotometer • 0Prepare a “blank” solution to calibrate the spectrophotometer by combing the following solutions in a cuvette or test tube. • 0Label the test tube as “blank” using labeling tape and a sharpie. o You may also write directly on the…

    Words: 2082 - Pages: 9
  • Bicarbte Buffer System

    1. List the steps you would take if you were to break a beaker with a pH 9 solution. First, once a spill occurs, ensure everyone (especially the lab instructor/advisor) around the area or in the lab knows about the occurrence. Second, it would be best to make sure that you have not been contaminated with the chemical, meaning that you should take care of yourself by washing the affected areas. Third, once your hands are dry, you should then make an effort to neutralize the solution of pH 9 as…

    Words: 1280 - Pages: 6
  • Principle Of Electrochemical Impedance Spectroscopy

    This is cleaned with water and ethanol followed by etching with 10:1 HF to remove the native oxides. This is directly dipped into 10% 10-undecanoic acid in deaerated toluene for 19h, with exposure to 352 nm ultraviolet light for photoactivated alkene insertion into Si-H bonds, which creates a carboxylate-terminated surface. The Au electrode was cleaned and immersed into 1mM 11-Mercaptoundecanoic acid and 50mM phosphate buffer for about 17h to form a carboxylate-terminated self-assembled…

    Words: 2102 - Pages: 9
  • Tem-1 B Lactamase Lab Report

    sodium phosphate buffer was pipetted into three 1.5 mL of microcentrifuge tubes each. 2. The three microcentrifuge tubes was labelled accordingly: T-37, T-45 and T-100 3. T-37 tube was placed in a heating block with the temperature set at 37oC, T-45 tube was placed in the 45oC heating block and T-100 tube was placed in the 100oC heating block. 4. Each tube was incubated for 5 minutes at their corresponding temperature heating block. 5. After, 10 µL of the enzyme sample was pipetted into each…

    Words: 1711 - Pages: 7
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