PH indicator

NOSIPHO ZUMA A00018164 BIO 120 DR HAYATU RAJI LAB 1: PH TEST FOR VARIOUS SUBSTANCES LAB PARTNERS: AMINA SULEIMAN, SEDOO ORJE-ISHEGH DATE: 27 SEPTEMBER 2016 Abstract The primary purpose of this experiment was to determine pH levels of various substances using different methods. Two techniques were used, the pH paper indicator and the pH meter. Out of ten substances that were used, five of them were found to be acidic, namely: hydrochloric acid, coke, toothpaste, milk, and acetic acid. Sodium hydroxide, buffer solution, bleach, and the detergent were found to be basic. Bottle water was found to be neutral. Substances are said to be acidic because of having pH levels lesser than 7, and those having pH greater than 7 are said to be basic. Some are neutral,…

acid or a base. One of the methods is by using an acid-base indicator or by identifying hydrogen or hydroxide ions in a substance’s chemical formula. Certain materials such as a plastic template, red litmus paper, blue litmus paper, phenolphthalein, universal indicator, and a pH chart can be used to help us distinguish acids and bases. Before beginning this experiment, a lab coat and goggles should be put on for safety considerations. After having appropriate clothing, begin setting up the…

The learning objective of the experiment performed was to determine the pH of solutions that were given by using litmus paper and pH machine. The Experiment was kind of successful except for an issue with one of the solutions. When we tried to figure out the pH by looking at the litmus paper; we were stuck between a pH of 6 and 7. The best choice was to choose a number in between 6 and 7. Based on the two methods used to find the pH the results were close to each other. In this experiment…

mannitol carbohydrate and produce gas. Majority of microorganisms obtain energy from either aerobic or anaerobic respiration, or through fermentation. The different types of fermentation pathways used by bacteria can aid in differentiating them among groups or species of bacteria because each pathway depends on the complement of enzymes available for the cell (Department of Biological Sciences, 2015). The identification of the bacteria can be determined through the characteristic end products of…

analyte. (using burette) Acid –base indicator: acid- base indicator is an indicator that determines the pH of a solution by changing its color due to H+ ion concentrations inside the solution. Indicator range: acid- base indicators change their colors when pH changes. However, they are useful in a specific pH interval. pH of a solution cannot be determined if pH is out of the interval of…

and bases; through this process an acid or base of known concentration neutralizes an acid or base of unknown concentration. The neutralization of an acid or base in acid-base titration can be measured with either a color indicator or a pH-meter. For this experiment we are using a color indicator. The indicator phenolphthalein is used in experiment 1 and starch was use as an indicator in experiment 2.Phenolththalein is a colorless in acidic solution and pink in…

It is important to note that the goal of this experiment was to find the pKa value of bromothymol blue, a pH indicator commonly used in titrations. [UNC, 2017] The pKa is where the number of moles of acid to conjugated base are equivalent. To find this, both Beer’s Law and spectroscopy were utilized in an attempt to make graphs to show just where this value occurred. According to Sigma-Aldrich, the pKa of bromothymol blue is approximately 7.10. [SIGMA, 2017] The final pKa found experimental was…

Scientists use a scale to measure how acidic or basic a liquid is. That scale is called a pH scale. The pH scale is a number from zero to fourteen. Accordingly, zero to seven liquids are considered acid. However, seven is considered a neutral, like distilled water. Therefore, eight to fourteen the liquids are considered basic. For instance, a pH of two is going to be an acidic liquid. Thus, a pH of twelve would be measured as a basic liquid. Additionally, a strip of litmus paper will…

These are the expected results of a negative indole test narrowing our genus options now to two potential bacteria. The Methyl Red test is biochemical test that involves the addition of the pH indicator methyl red to an inoculated tube of MR-VP broth. MR-VP (Methyl red and Voges-Proskauer) broth is used as two parts of the of IMViC tests. MR-VP broth is a medium that both the methyl red and the Voges-Proskauer tests can be performed. This broth is considered to be a simple broth and contains…

well-isolated colony “pure culture” and tested using a gram stain to determine morphology, arrangement, size and verify purity again. Once purity has been verified, a portion of the same colony is transferred to a two separate nutrient agar slants and incubated. This will be the pure culture working stock to be used for future testing and the other slat will be used as a reserve stock if needed. Using the working stock, the following tests are preformed aseptically: oxidase test and the reagent…