Oxidase Test In Microbiology

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Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also to figure out if the patient have contracted a new disease causing microorganism, which then need to be studied to find a way to contain it and potentially find a treatment or cure for it before it spread to effecting other people and cause more damage …show more content…
The following tests are done to differentiate and eliminate possible organisms. Methyl Red test and the indicator is pH indicator, Voges-Prokeur test and the indicators are barrits A and barrits B, citrate test, nitrate broth and add reagents A+B and maybe powdered zinc. The citrate, MR and nitrate tests were repeated twice at different times to confirm the results. At this point, the possibilities were down to two genera. To help eliminate one of them the following tests are done: lactose fermentation test (was repeated to confirm result on a different day), SIM test and Kovac’s reagent is used (was repeated twice to confirm results), urease hydrolysis test (was repeated twice to confirm results), catalase test using hydrogen peroxide, gelatin hydrolysis and casein hydrolysis tests. This eliminated the possibilities to only one genus. To confirm the results a multiple system test “Enterotube II” is done. Bergey 's manual of determinative bacteriology is used to determine the unknown using my results. All the information and details about the procedure of the tests mentioned in the method section can be found in the Leboffe lab

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