PH indicator

Interpretation and Evaluation: Rinsing Process: When performing a volumetric analysis all equipment to be used must be washed with deionised water. This is done in order to remove anything that contaminate your solution and have an effect on the results. It is important that deionised water is used and not regular tap water, this is because tap water could itself contains many unwanted impurities such salts, other solids and bacteria. If these were to contaminate the solutions to be used it…

that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic pyrophosphatase. ATP was added, whose final concentration was 1.5mM and the rest of the volume was the…

Lactate dehydrogenase was assayed according to the method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5.…

can be determined by the white vinegar with the known concentration of sodium hydroxide from the previous lab. (Eq 2). CH3CO2H(aq) + NaOH(aq) = CH3CO2Na(aq) + H2O(l) (Eq 2) The purpose of indicator used to determine the end point of the reaction when both the reactants and product are colorless. The experiment goal of the lab is to determine the concentration of acetic acid (CH3CO2H) in the commercial white vinegar brand. Procedure and…

REFLECTIONS FOR LAB 4- FRICTION Lab 4.1 to 4.3 is about all the different frictions. We did 3 different labs. 4.1 was pulling a block across different surfaces and finding the difference in friction. 4.2 was about pulling multiple blocks across different surfaces and finding the difference in friction. 4.3 was about pulling blocks but in a bigger surfaces area. In Lab 4.1 we had to pull different blocks across different surfaces. The surfaces were a tabletop, wax paper, paper towel, fine…

with phenolphthalein, and a modern titration, with a pH probe, were executed. Through the use of the equation for molarity and stoichiometry, the molarity of citric acid was able to be calculated from V_NaOH, which was found in the traditional titration by subtracting Vi from Vf and…

Calculations- Representative Fraction (RF): 0.92cm / 8.95cm = 0.10 Limitations- Ruler: +/- 0.05 cm One possible limitation for this experiment was the contamination of the chromatography paper by foreign amino acids. Any amino acids that our gloves came in contact with throughout the lab transfered over to the chromatography paper and appeared as purple spots, mostly on the border of it. Conclusion- The amino acids in unknown mixture A were Glycine and Valine. The amino acids in unknown…

Conclusion: I predicted that liquid A was glycerine due to a calculated density of 1.38 g/mL. However I was wrong the actual density of liquid A was 1.38 g/mL and the substance was actually corn syrup. Therefore the percent of error for the density of liquid is 1%. For liquid B I had predicted that its substance would be Glycerine due to the calculated density of 1.19 g/mL. I was incorrect for the density of liquid B which was actually 1.26 g/mL, but because the calculated and actual densities…

The two main purposes of the lab were to determine the percent by mass of acetic acid in vinegar and to determine the molar concentration of a strong acid. In both parts of the experiment a volumetric analysis was used. This is primarily the aid of volumetric glassware in order to determine specific measurements and calculations. Potassium hydrogen phthalate was used as the primary acid standard to determine the molar concentration of the sodium hydroxide solution in part one of the experiment.…

The objective of the lab was to determine the densities of known and unknown metal solids and liquids. For the liquids, initially, an Electronic Scale was placed onto Work Bench, and it was zeroed. An empty 50 mL Graduated Cylinder was placed onto the scale, weighing 54.00 grams. In increments of 10 mL. Until the volume was 30.00 Ml, 10.00 mL of water was poured into the Graduated Cylinder. In between each increase of 10.00 mL, the total volume and mass were recorded. For the known metals, the…