Growth medium

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    will have a purple butt, and if negative it will have a yellow butt. The Sulfide Indole Motility (SIM) blue cap tube is a semisolid agar and it is also used to determine sulfide production, indole formation and motility in Gram negative bacteria. We will be adding a couple of drops of Kovacs reagent following the incubation of the unknown organism. The indole combines with the reagent and if positive it produces a red layer on top of the medium. If the medium has a hazy growth throughout, then is positive for motility and if it produces Hydrogen Sulfide (H2S) a black precipitate is produced. Some bacteria can produce large amounts of stable acids and others produce a smaller amount. In order to know which ones produce more or less we will do the Methyl Red Test (MR) yellow cap tube. We will be adding Methyl Red to the broth after incubation to observe fermentation and it will turn red if pH is below 4.4 (positive result) and will remain yellow if ph is above 6.0 (negative result). The Triple Sugar Iron (TSI) red cap tube is a differential medium. If the organism is able to fermentate any of the three sugars the slant is purple and/or has a yellow butt (negative). If the black precipitate is present, then there was fermentation and H2S was produced. The ability to clear boxylate lysine will produce a purple butt (positive). If the fermentation produced gas the entire slant may be raised above the bottom of the tube. The Simmons citrate green cap tube tests the ability…

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    purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable growth when applied. The different medias will either promote or inhibit the growth of the…

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    and AuNPs against the eight chosen S. aureus isolates (four MRSA+VRSA and four MRSA+VSSA) were analyzed through determination of their minimum inhibitory concentrations (MIC) values, minimum bactericidal concentrations (MBC) values, and MBC/MIC ratio by broth micro-dilution method using 96-well micro-titer plates. To each 50 μL of the antibacterial agent dilution, 50 μL of adjusted bacterial concentration inoculum (5 × 105 CFU/mL) were added in the 96-well micro-titer plates, the growth control…

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    Materials and Methods Overview The whole study consists of four phases. To start with, the suitable media need to be identified for the growth of Av. paragallinarum. Secondly, the media used in disc diffusion and broth dilution testing need to be validated. This validation process is completed by performing disc diffusion and broth dilution testing of quality control strain on candidate agar or broth. Then, the antimicrobial sensitivity of Av. paragallinarum can be tested on validated media, by…

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    Catalase Test Essay

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    perform a verity of other environmental requirement/reaction tests. This Includes incubation of the gram positive bacteria on a 6.5% NaCl medium, BHIA (Brain Heart Infusion Agar) medium and a Blood agar medium. Interpretations of these results are as follows. The NaCl 6.5% medium was used to test the bacteria’s ability to survive in a salt rich environment. Most organisms are unable to survive in this type of environment because it is hostile. This test allows us to confirm and narrow down our…

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    Probiotic Cream Case Study

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    products of milk which date back several thousand years (Sarrela, 2000). According to the definition of the Food and Agriculture Organization of the United Nations, probiotic foods are a group of food products containing one or a mixture of beneficial live bacterial cultures which their consumption leads to a balance in the gut flora and finally the health of humans (FAO/WHO, 2001). More than 500 probiotic food products have been introduced in global markets during two recent decades (Anonymous,…

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    TLC Semi Quantitative Dpph

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    2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

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    Unknown Bacteria

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    doing the catalase test she had recommended that I do a mannitol salt agar test or a Blood agar test for my final test, which would help determine between Streptococcus pneumoniae, Staphylococcus epidermidis. I decided to go along with the mannitol salt agar method and again with my inoculated loop I grabbed some of my unknown #15 and I had applied it to aseptic technique to the agar plate. While waiting for my results to appear, this test will show us whether or not the unknown #15 ferments…

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    Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…

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    Introduction: Sampling the microbial content of the environment is a recommended practice for hospitals and clinics. Information obtained by environmental sampling has great value in the evaluation of problems relating to housekeeping procedures, disinfectants, air distribution and filtering, traffic control and the influence of specific equipment on environmental contamination. The purpose of this practical is to assess the effectiveness of some cross-infection control measures commonly…

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