Growth medium

will have a purple butt, and if negative it will have a yellow butt. The Sulfide Indole Motility (SIM) blue cap tube is a semisolid agar and it is also used to determine sulfide production, indole formation and motility in Gram negative bacteria. We will be adding a couple of drops of Kovacs reagent following the incubation of the unknown organism. The indole combines with the reagent and if positive it produces a red layer on top of the medium. If the medium has a hazy growth throughout, then is positive for motility and if it produces Hydrogen Sulfide (H2S) a black precipitate is produced. Some bacteria can produce large amounts of stable acids and others produce a smaller amount. In order to know which ones produce more or less we will do the Methyl Red Test (MR) yellow cap tube. We will be adding Methyl Red to the broth after incubation to observe fermentation and it will turn red if pH is below 4.4 (positive result) and will remain yellow if ph is above 6.0 (negative result). The Triple Sugar Iron (TSI) red cap tube is a differential medium. If the organism is able to fermentate any of the three sugars the slant is purple and/or has a yellow butt (negative). If the black precipitate is present, then there was fermentation and H2S was produced. The ability to clear boxylate lysine will produce a purple butt (positive). If the fermentation produced gas the entire slant may be raised above the bottom of the tube. The Simmons citrate green cap tube tests the ability…

and AuNPs against the eight chosen S. aureus isolates (four MRSA+VRSA and four MRSA+VSSA) were analyzed through determination of their minimum inhibitory concentrations (MIC) values, minimum bactericidal concentrations (MBC) values, and MBC/MIC ratio by broth micro-dilution method using 96-well micro-titer plates. To each 50 μL of the antibacterial agent dilution, 50 μL of adjusted bacterial concentration inoculum (5 × 105 CFU/mL) were added in the 96-well micro-titer plates, the growth control…

purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable growth when applied. The different medias will either promote or inhibit the growth of the…

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

Vocabulary 1. Inoculum: a material used for inoculation. 2. Loop (the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and…

perform a verity of other environmental requirement/reaction tests. This Includes incubation of the gram positive bacteria on a 6.5% NaCl medium, BHIA (Brain Heart Infusion Agar) medium and a Blood agar medium. Interpretations of these results are as follows. The NaCl 6.5% medium was used to test the bacteria’s ability to survive in a salt rich environment. Most organisms are unable to survive in this type of environment because it is hostile. This test allows us to confirm and narrow down our…

To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…

products of milk which date back several thousand years (Sarrela, 2000). According to the definition of the Food and Agriculture Organization of the United Nations, probiotic foods are a group of food products containing one or a mixture of beneficial live bacterial cultures which their consumption leads to a balance in the gut flora and finally the health of humans (FAO/WHO, 2001). More than 500 probiotic food products have been introduced in global markets during two recent decades (Anonymous,…

formation and this show that E. coli is able to use the nutrient agar and growth into a unit of colonies. iv. The Brain Heart Infusion (BHI) agar plate is use for growing bacteria in the plate. All three of the bacteria are successful grown on the plate indicating by the wider appear of streak mark. The MacConkey agar plate was both a selective medium with the bile salt and crystal violet and differential on the ability of break down lactose and pH indicator. The E. coli turn pink indicating…

were localized in the cytosol, that it might be able to bind to DNA, and that it also existed as a dimer. These findings were crucial, as they were the one of the main reasons why Dr. Liu decided to publish another paper on Lsr2, in which he unveiled the true function of Lsr2 Mtb in 201012. Before diving into his 2010 paper, let's discuss some implications of his 2006 paper. All the results from the 2006 publication indicate that Lsr2 inhibitors may be able to disrupt proper biofilm formation.…