Growth medium

Objectives: The purpose of this project is to identify two separate microbial organisms from a given list, which are to be inoculated from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to…

Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…

37 °C for 48 hours. This process was done on October 11th and repeated on October 18th. The growth was observed and recorded in Table 4. A sterile inoculating loop was used to quadrant streak samples of Staphylococcus epidermidis and Micrococcus luteus on agar plates. This process was done on October 11th and repeated on October 18th. The plates were incubated at 37 °C for 48 hours. The resulting growth was recorded in Table 6. A sterile inoculating loop was used to spot inoculate Escherichia…

Introduction : Enterotube II is used to identify multiple tests in one tube. twelve different conventional media plus a self-contained inoculating wire. The twelve test are for glucose and gas, then lysine, ornithine, H2S and indole, adonitol, lactose, arabinose, sorbitol, dulcitol, phenylalanine (amino acid), urea, and citrate (No VP test require). These medias performs 15 different biochemical tests that will determine the genus and the species of gram negative bacteria. This test will help…

The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…

in diameter and belong to the Enterobacteriaceae Family. E. aerogenes have capsule with flagella which enable them to be motile. They are facultative anaerobes which mean they are able to survive with or without the presence of oxygen. The optimum growth temperature is range from 30oC to 37oC which classify as a mesophile. It is commonly associated E. aerogenes with nosocomial infection and they are acknowledged as opportunistic pathogens. Several infections cause by E. aerogenes including…

We began by examining the transformed bacteria on the LB/amp/ara plate and the LB/amp plate under UV light to find a single colony expressing the GFP gene. We obtained two culture tubes with 2 ml growth media and transferred the glowing colony from the LB/amp/ara plate into one tube and the non-glowing colony from the LB/amp plate into the other tube. Then, we shook both tubes vigorously by hand for thirty seconds, before placing them in a shaking…

Petri plate as no bacteria colony was observed on the surface of agar plate. The stool and sink in the laboratory was probably disinfected with 70% alcohol and anti-bacterial agent before the experiment was done resulting in the lack of microorganism growth on the agar surface. The bacteria might also be obligate anaerobes that cannot live in the presence of…

a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment lactose. A Gram stain was then performed to confirm that bacteria #12 was indeed gram-negative and also showed that…

Speech #1 Edward R. Murrow, a CBS reporter and war correspondent delivered a report from Buchenwald, Germany on April 16, 1945. He delivered this dialect upon seeing the atrocities committed by the Germans towards the Jews. He addresses the American people, describing the scene he had witnessed at this labor camp, which he found the scene to be so unbelievable that he is rendered speechless many times through out his speech. Murrow’s outrage is so apparent through-out his account, that it is…