Growth medium

Materials and Methods Overview The whole study consists of four phases. To start with, the suitable media need to be identified for the growth of Av. paragallinarum. Secondly, the media used in disc diffusion and broth dilution testing need to be validated. This validation process is completed by performing disc diffusion and broth dilution testing of quality control strain on candidate agar or broth. Then, the antimicrobial sensitivity of Av. paragallinarum can be tested on validated media, by…

we tested the growth on Mannitol Salt Agar (MSA). To conduct this experiment, we needed to first create another live liquid sample using an isolated colony from the LB agar, and then transfer some of this culture into a MSA petri dish. We then added five sterile beads and shook them around the agar to distribute the bacterial cells. Once this was complete, we allowed the petri dish to incubate at 37°C for two days to allow the formation of new colonies. We then checked for growth/color…

In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

The objective of this activity was to use the knowledge that we obtained throughout the semester, with the goal to correctly identify two unknown bacteria. Organism number thirteen is Escherichia coli. On the first day, I conducted a gram stain and noticed that the bacteria were pink, meaning that the bacteria are gram negative. I also recognized that the bacteria were rod shaped therefore they were bacillus. I also noticed that the arrangement appeared to be single celled. I also put the…

Streptomyces and Antibiosis From our group, Group 2, nutrient agar plates inoculated with E. Coli, (Images 1.1,1.2), (Images 2.1,2.2), (Images 3.1,3.2) no antibiotic activity was detected. This could be a result of the putative colonies chosen, not being Streptomyces. All selected colonies were white, or pigmented rink, raised and rough. One (1) and three (3) were inoculated with the putative Streptomyces colonies. Plate 2 was inoculated with the control Streptomyces colony. Although it was…

Introduction: Sampling the microbial content of the environment is a recommended practice for hospitals and clinics. Information obtained by environmental sampling has great value in the evaluation of problems relating to housekeeping procedures, disinfectants, air distribution and filtering, traffic control and the influence of specific equipment on environmental contamination. The purpose of this practical is to assess the effectiveness of some cross-infection control measures commonly…

containing 9mL water using a pipette. Four MSA and four PEA plates were used for isolation. PEA plate number 1 was divided into three parts and was used to isolate all of the samples obtained from the skin by rolling the cotton swabs on the surface of the medium. PEA plate number…

mannitol, which will make it change to the color yellow. If it does not change to yellow that just simply means that the unknown #15 can grow on mannitol salt agar and handle the salt. My unknown did not change color at all, but it definitely shown some growth on the plate, which leads me to negative for Staphylococcus…

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…