Bacillus

DISCUSSION Unknown #297 was collected from a urine sample from a patient with a kidney infection, named Doris. There have been several tests that were performed at the University of Illinois clinical Laboratory to identify the unknown. Unknown #297 was identified to be Bacillus subtilis with the various tests that were conducted. Most of the results that were observed in the laboratory matched the expected results for the bacterium. All of the morphological characteristics results matched expect in one test. There was a discrepancy in the motility test, where the test was observed to be negative. According to Microbewiki2, the test was supposed to be positive. The discrepancy might have been due to the error within the aseptic technique that was performed. When the tube was inoculated the needle might have been too hot, which might’ve killed the bacteria, to cause it to be non-motile. The starch hydrolysis test was observed to be negative. However, brown stated that the test for Bacillus subtilis should come out positive. When the starch plate was observed, there was a lot of growth, and there was only a small circle that was hydrolyzed in the middle of the plate.…

1. Summary of how I identified Bacillus cereus. a. First, I performed the gram stain test, I then went on to do the spore stain. b. The results I obtained from the gram stain were gram positive rods, I could tell they were positive by the purple color to them and the rod shape by the comparison provided in the lab manual (Wilkosz, Peterson). After I confirmed the results I performed the spore stain, which indicated that my sample was positive so it contained endospores. The sample should have…

Bacillus anthraces is an intense irresistible malady brought on by Bacillus anthraces, a spore shaping, gram-positive bacillus. Related malady happens most much of the time in sheep, goats, and dairy cattle, which get spores through ingestion of polluted soil. People can get to be tainted through skin contact, ingestion, or inward breath of B. anthraces spores from contaminated creatures or creature items (as in "fleece sorter's infection" from introduction to goat hair). Individual…

Biology assignment Introduction ? There are many bacteria present in the world that affect people on a daily basis. There are any antiseptics available on the market to help combat the war against pathogenic bacterium. A common type of bacteria includes bacillus subtillis. ?B. subtillis is a bacterium found on the skin, in the digestive tract, in epithelial wounds, on extremities of the human body, in livestock and soil. Because B.subtiliis is ubquituous, it has developed adaptive strategies…

their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain individually isolated colonies. The gram-staining procedure was used to determine organismal morphology, resulting in a gram-positive cocci and gram-negative bacilli. A sucrose utilization test was performed and narrowed the possibilities of the gram-negative bacilli to Proteus vulgaris and Enterobacter cloacae. Then a Methyl Red and Voges-Proskauer (MR-VP) test was done to confirm the identity of the…

In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…

2. Materials and Methods: The keratin was extracted from Chicken feather which were collected from the local market of Raipur (21.14°N 81.38°E) Chhattisgarh, India. The 91% food grade alginate (sodium salt form) was purchased from Loba Chemie. The β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria…

Bacillus anthracis is the causative organism of the serious infectious disease, called anthrax. There are three manifestations of this disease. The manifestations include gastrointestinal anthrax, inhalation anthrax, and cutaneous anthrax. This research will explore the morphology, virulence factors, reservoir, transmission, and portal of entry of B. anthracis. The pathogenesis will also be reviewed, including symptoms, how B. anthracis causes these symptoms, the possible complications of the…

Anthrax is caused by spore forming bacterium. It mostly affects animals. Humans can be infected if come in contact with infected animals. It was most common with sheep and cattle. In 1881, Louis Pasteur focused his attention on anthrax. Louis Pasteur performed public experiments in which he injected one group of animals with an anthrax vaccine he developed (9). Pasteur gave 25 shots of anthrax vaccine he had created with a weakened anthrax bacteria (8). Pasteur gave both rounds of the vaccine…