Chromatography

Strasberg, P.M., and Lowden, J.A. (1982) The assay of glucocerebrosidase activity using the natural substrate. Clin. Chim. Acta, 118 (1), 9–20. 36. Grabowski, G.A., and Dagan, A. (1984) Human lysosomal β-glucosidase: Purification by affinity chromatography. Anal. Biochem., 141 (1), 267–279. 37. Maret, A., Salvayre, R., Potier, M., Legler, G., Beauregard, G., and Douste-Blazy, L. (1988) Comparison of Human Membrane-Bound β-Glucosidases: Lysosomal Glucosylceramide-β-Glucosidase and Non-Specific…

Inborn errors of metabolism are a group of rare disorders characterized by metabolic pathway abnormalities. One such disorder is Maple Syrup Urine Disease (MSUD) otherwise known as Branched-chain Ketoaciduria, a dangerous condition in which over saturation of amino acids in the blood can lead to toxicity progressing to encephalopathy, neurodegeneration, coma or respiratory failure (Haldeman-Englert, 2015). MSUD results from a malfunction of the branched-chain alpha-ketoacid dehydrogenase complex…

1. Describe two scientific theories relating to the evolution of the chemicals of life A scientific theory is an explanation based on observations, reasoning and experimentations, especially one that has been tested and confirmed as a general principle helping to understand, explain and predict natural phenomena. A scientific theory must be based on careful and rational examinations of the facts. There are 5 major theories that account to the evolution and origins of chemicals of life. However…

INTRODUCTION In the recent advancement of targeted therapeutics that was coupled with new approaches in target identification have accelerated the need to design small compounds with drug like properties [1]. Quinoxaline is well known for its broad coverage in the field of medicine as well as for its application in the pharmaceuticals as they have shown wide range of biological properties such as antimicrobial [2], antibacterial [3], antitubercular [2, 4], antiprotozoal [5] , anti-candida [2, 6]…

Literature review Polymerisation of Cyclo pentene, Cyclo hexanes, Substituted Cyclo pentene and Cyclo hexanes using different catalyst Introduction:- Polymer is a word which is derived from Greek words “poly” which means many and “mer” which means parts. Polymers are substances which have large number of repeating structural units repeated in the same type of linkage. The chemistry that deals about polymers is called polymer chemistry and the reaction is called as polymerization. Polymer…

A common topic in high schools of late has been the use of drugs and steroid use among athletes. High schools have the ability to test athletes for the safety of the students. Although some parents and students believe that drug testing students in high school causes problems that can be avoided by not testing them. In high school athletics, people have proposed to drug test athletes for the safety of the athletes; however, other people believe that they should not be drug tested because it…

Grabowski, G.A., and Dagan, A. (1984) Human lysosomal β-glucosidase: Purification by affinity chromatography. Anal. Biochem., 141 (1), 267–279. 37. Maret, A., Salvayre, R., Potier, M., Legler, G., Beauregard, G., and Douste-Blazy, L. (1988) Comparison of Human Membrane-Bound β-Glucosidases: Lysosomal Glucosylceramide-β-Glucosidase and Non-Specific β-Glucosidase…

A COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF ALOE VERA , SESAME OIL AND TEA TREE OIL AGAINST E. faecalis - AN IN-VITRO STUDY” INTRODUCTION Medical microbiology entered a new era towards the end of the nineteenth century. It was during these golden years that path-breaking work of Pasteur, Koch and others in the development of broth and agar media led to the ability to grow a majority of pathogenic micro-organisms which were deemed uncultivable…

Mesalazine and Rifaximin use for Inflammatory Bowel Disease. Mesalazine is used as in anti-inflammatory agent, Steroidal. Rifaximin is used in Gastrointestinal Agents, Anti-infective agent. The use of Rifaximin in combination with Mesalazine has been proved to provide beneficial effect in inflammatory bowel disease. The mechanism of Mesalazine and Rifaximin is quite different. Mesalamine and Rifaximin are two different types of drugs offering some symptomatic relief to the IBD patients.…

Silica based columns with different cross linking’s in the increasing order of polarity are as follows: ß------- Non-polar----------moderately polar--------Polar----------à C18< C8< C6< Phenyl < Amino <Cyano< Silica  In reverse phase chromatography, hydrophobic interaction between drug molecule and the alkyl chains on the column packing material.  Column is selected based on solubility, polarity & chemical differences among analytes and Column selected: i.e.…