Gas chromatography (like any other chromatography) is a separation technique in which the mobile phase is a gas. This technique is applied for the separation of volatile substances. This method separates a mixture into its components and is used to measure the concentration as well as it identifies the components.
GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated. Each peak in a chromatogram represents a different component of the original mixture. Time can be used to identify and peak size for detection the amount.
Compounds that are mixed together are separated by GC and the chemicals are fragmented into a unique spectrum by MS. A sample is dissolved into a specific liquid which is then injected into a stream of gas. Generally used gases are nitrogen or hydrogen. Then the gas containing the sample passes through the column where …show more content…
Separation is based on relative affinities of sample components, towards the stationary phase. The component having more affinity towards the adsorbent will travel slower and the components having less affinity towards the stationary phase will travel faster. Since no two components have same affinities, they get separated.
In this technique a small volume of a sample is inserted into a tube, called stationary phase. Sample molecules moves through the column or packed tube along with a liquid mobile phase. High pressure pumps forces the liquid phase through the column. Many chemical and physical interactions take place in column, between the molecules and column packing material. In this way components are separated. These separated components are detected by a detector that measures the quantity. An output is generated in the form of a chromatogram.
GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated. Each peak in a chromatogram represents a different component of the original mixture. Time can be used to identify and peak size for detection the amount.
Compounds that are mixed together are separated by GC and the chemicals are fragmented into a unique spectrum by MS. A sample is dissolved into a specific liquid which is then injected into a stream of gas. Generally used gases are nitrogen or hydrogen. Then the gas containing the sample passes through the column where …show more content…
Separation is based on relative affinities of sample components, towards the stationary phase. The component having more affinity towards the adsorbent will travel slower and the components having less affinity towards the stationary phase will travel faster. Since no two components have same affinities, they get separated.
In this technique a small volume of a sample is inserted into a tube, called stationary phase. Sample molecules moves through the column or packed tube along with a liquid mobile phase. High pressure pumps forces the liquid phase through the column. Many chemical and physical interactions take place in column, between the molecules and column packing material. In this way components are separated. These separated components are detected by a detector that measures the quantity. An output is generated in the form of a chromatogram.