Chromatography

Objective The objective of the experiment was to determine the purity and components of a mixture of fluorescein and bromonitroindoline, as well as separate them, through the process of thin layer chromatography. Procedure Part A- Separation of Compounds by Thin Layer Chromatography • Three 100 mL beakers and watch glasses were cleaned in preparation for this experiment. Three silica gel plates were acquired from the instructor, and to each plate a line was drawn 5 mm from the edge of the…

pure octane. The distillation could be completed because part of the solution, hexane, had a lower boiling point than the other, octane, allowing it to evaporate out. Throughout the experiment, four samples were taken and tested by gas chromatography. Gas Chromatography, or GC, was used to analysis the components of each fraction taken during the experiment. Upon looking at the data, the presence of hexane in the fractions decreased, whereas, the presence of octane increased. This was due to the…

The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate…

A small burette funnel was filled with column packing solvent at mobile phase, high boiling petroleum, in this case hexane (and then 10%acetone hexane mixture), and Alumina as stationary phase. When adding the sand and the alumina they have to be first mixed with the hexanes in order not to disturb the column. The Lycopene is Red- Orange and more attracted to alumina due to its high degree of Unsaturation, which can be expected to be eluted after Yellow-Orange Carotene pigments. And the other…

Introduction According to Bio-Rad, Alamar Blue is a “cell viability assay reagent which contains the cell permeable, non-toxic and weakly fluorescent blue indicator dye called resazurin.” (Bio-Rad, n.d.). Meaning that the resazurin is going to be using an oxidation-reduction (REDOX) indicator that will undergo a color change due to the response of the cellular metabolic reduction. “The reduced form resorufin is pink and highly fluorescent, and the intensity of fluorescence produced is…

Twenty Korean barley cultivars (Geongang, Gwangan, Nackyoung, Dahyang, Daejin, Saegang, Boanchal, Keunal, Saessal, Saechalssal, Sujung, and Boanchalssal) were selected in this research. All the cultivars were developed by the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea and were grown during 2013 in the experimental fields of the NICS, Milyang, Gyeongnam. After harvesting, barley leaves were immediately washed with clean sterile water and then…

Emulsions are two-phase systems, a mixture of two or more liquids that are normally immiscible. There are two phases in the system, in which one is the continuous phase and the other one is the dispersed phase. Types of emulsions [38] Oil in water (o/w): in which oil is the dispersed phase and water is the continuous phase. Water in oil (w/o): in which water is the dispersed phase and oil is the continuous phase. Emulsions have a cloudy or opaque appearance because particles scatter light…

EXPERIMENTAL RESULTS The present study was undertaken to formulate Solifenacin succinate immediate release tablets. The study involves preformulation studies of drug excipients, formulation and processing development along with evaluation of tablets made with the optimized formulation. Finally tablets were evaluated by in-vitro methods. 6.1. Drug excipient compatibility study Table 1. Results of compatibility study S.No. Name of Excipient Ratio of API/ Excipient Initial…

ABSTRACT Flakiness & Elongation index is one of the most prominent criteria that govern behavior and performance of aggregate in the bituminous mixes. The strength and serviceability requirements of bituminous mixture such as Stability, Flow, Voids in Total mix (VTM), Voids Filled with Bitumen and (VFB) highly depend on the physical properties of aggregates. This study conducted by observing the effect of flakiness & elongated index by adding different percentages from 0% to 50% of flaky &…

Stereochemistry conformation is how molecules are orientated in space. There are two basic types of isomers: constitutional or stereoisomers. Constitutional isomers gave the same molecular formula but different connectivity, while stereoisomers have the same connectivity but a different orientation in space. Stereoisomer has two subunits called enantiomers and diastereomers. Enantiomers are molecules that are mirror images, while diastereomers are not mirror images. Stereochemistry focus…