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  • Plasmid Lab Report

    Addition of the Plasmid vs the Expression of the Added Plasmid Introduction The earliest example of genetic engineering was performed by Gregor Mendel in 1865, when Mendel performed artificial selection on a population of pea plants in the monastery where he lived ( Once the technological advancements of the 20th century was introduced to the science field, genetic engineering took on a whole new light. Scientist could now transform organisms in ways that no one ever could. E. coli is the perfect test subject for this lab because, as stated by Pearson schools, “E. coli reproduce very rapidly; a single microscopic cell can divide to form a visible colony with millions of cells overnight. Like…

    Words: 1520 - Pages: 7
  • Plasmids In Biological Research

    Introduction Plasmids, circular pieces of DNA that can replicate independently from the host cell’s DNA, have been extensively used to transform cells in biological research to study the effects of particular genes of interest (Lau et al., 2013). A plasmid’s basic components include a replication origin, a DNA marker, and a multiple cloning site, which are sufficient for the plasmid to replicate itself and help the transformed cell to exhibit characteristics indicating successful…

    Words: 891 - Pages: 4
  • Pglo Plasmid By E. Coli

    coli, have their DNA in the form of a plasmid. A plasmid is a circular ring of DNA. The plasmid intended to transform the E. coli colonies for the purposes of this study was the pGLO plasmid. The pGLO plasmid contains three main genes: one for GFP (green fluorescent protein), one for ampicillin (an antibiotic) resistance, and one that codes for the sugar arabinose (Bassiri 2011). The process used to transform the E. coli bacteria with the pGLO plasmid was by means…

    Words: 1468 - Pages: 6
  • Plasmid DNA Transformation Lab Report

    limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral pH.…

    Words: 1536 - Pages: 6
  • E. Colo Transformation Lab Report

    exposed to an extracellular plasmid of DNA. It involves introducing the E. coli and allowing it to adapt to the DNA of a new environment. The E. coli must be placed in a tube, into an ice bath, causing the cell to shrink. From there it is transferred to a preheated bath, where the cell is heat shocked, allowing the plasmids ' to enter the cell at a rapid pace, once the cell expands. After the tubes are incubated, they were transferred to six agar plates. There were two types of agar plates, one…

    Words: 722 - Pages: 3
  • Change For A Better Span Of Life Essay

    diagnosis for many genetic conditions…thanks to advances in single-cell diagnostics and fertilization technology, embryos can now be created in vitro, then, only those embryos that are not affected by a specific genetic illness can be selected and implanted in the woman’s uterus” (Simmons). This process is called preimplantation genetic diagnosis, otherwise known as PNG. Genetic engineering has been done for those individuals whom have diabetes. Scientists were able to create insulin so that…

    Words: 1082 - Pages: 5
  • Plasmid Synthesis

    Transforming Genetic Material To E. Coli Using Plasmids Proteins are created within the body through a process known as central dogma. This process is the transcription of DNA to RNA and the translation of RNA to proteins. The type of protein created relies on what type of messenger RNA or mRNA that was produced during the transcription of DNA. In our experiment, we looked at the specific LacZ gene that codes for beta-galactosidase, which is a protein/enzyme that can break down lactose into its…

    Words: 1599 - Pages: 6
  • Genetic Centriculation Lab Report

    Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist…

    Words: 1324 - Pages: 5
  • Pglo Transformation Lab Report

    bacterium cell walls more permeable and a heat shocking method to introduce the pGLO plasmid in the E.coli bacterium so that they may exhibit ampicillin resistance. The Goal of the experiment was to observe whether or not, given one of the four specific conditions, the pGLO plasmid would be able to transcribe itself into the E.coli bacterium. If the cells were successful in up-taking the plasmid, then they would inherently become resistant to ampicillin, be able to grow into colonies, and…

    Words: 1027 - Pages: 5
  • Genetic Transformation Lab Report

    The bla gene codes for beta lactamase which promotes antibiotic resistance for drugs classified as being a part of the penicillin family. In the pGLO plasmid there is a unique gene regulation system which controls the transcription of the green fluorescent protein. In order for the GFP to be expressed, arabinose sugar must be present. Arabinose sugar reacts with the AraC protein, and this reaction initiates transcription of the GFP gene. The purpose of this experiment is to see whether bacteria…

    Words: 1643 - Pages: 7
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