Cells exposed to -DNA did grow, but did not glow. The plate with -DNA/+Amp grew and didn’t glow; however, the cells in that plate shouldn’t have grown or glowed because only cells with +DNA contained the pGFP. In the plates labeled +DNA/+Amp and +DNA/+Amp/+IPTG nothing grew or glowed despite the presence of ampicillin and the glowing gene, which means the bacteria did not pick up either of the genes. In the experiment, the resistant ampicillin gene and the gene for green fluorescent protein was tested. After incubating the plates, it became clear how DNA can be transferred between bacteria through the process of transformation. In total conclusion, the lab intended to show that the gene for B-lactamase promotes growth even in the presence of ampicillin, and the promoter protein IPTG codes for a gene that makes cells
Cells exposed to -DNA did grow, but did not glow. The plate with -DNA/+Amp grew and didn’t glow; however, the cells in that plate shouldn’t have grown or glowed because only cells with +DNA contained the pGFP. In the plates labeled +DNA/+Amp and +DNA/+Amp/+IPTG nothing grew or glowed despite the presence of ampicillin and the glowing gene, which means the bacteria did not pick up either of the genes. In the experiment, the resistant ampicillin gene and the gene for green fluorescent protein was tested. After incubating the plates, it became clear how DNA can be transferred between bacteria through the process of transformation. In total conclusion, the lab intended to show that the gene for B-lactamase promotes growth even in the presence of ampicillin, and the promoter protein IPTG codes for a gene that makes cells