Plasmid

coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was conducted in order to transform E.coli into an antibiotic-resistant bacteria by introducing a plasmid, lux and pUC18, containing a gene for the resistance to Ampicillin. The procedure had four basic steps: treating bacterial cells with CaCl2 solution in…

The Effects of pGLO Plasmid Injection on Prokaryotic Gene Expression. By Kush Patel 3rd Period, AP Biology, February 12, 2024. Lab Partners: What is the difference? Brody Bonsor Andrew Zhang Marianne Baquero Clarie Gabrielson Abstract: In the pGLO lab, E. coli bacteria were genetically modified using a plasmid that contained three key genes: araC, GFP, and ampicillin resistance. Through a process of heat shock and incubation, the pGLO plasmid was successfully introduced…

base pairs for the PGEX-KG (original) plasmid and PGEX-KG SAW1 (clone gene) is different. PGEX-KG- Saw1 has 794 bp more than original plasmid. The single digestion for both PGEX-KG and PGEX-KG-Saw1 will make a single cut in their respective restriction sites. The clone and original plasmids will become linearized and the PGEX-KG-Saw1 will be longer than PGEX-KG because of the insertion of Saw1. However, the insertion Saw1 can be isolated from the clone plasmid by doing double digestion with…

LB plates, colonies on the “+ Plasmid “ kanamycin plate, and colonies two of the “+ plasmid “ Ampicillin plates. This experiment did not come out as predicted due to the lack of growth from some of the groups. Our results consisted of brown lawns on all the LB plates , no growth on any of the plates for plasmid one, one green colony for plasmid two on the edge of the LB/Amp plate, and two white colonies on the edge of the the LB/Amp plate. After testing the plasmids for the green fluorescent…

Plasmid Interference in Regulation of the Type III Secretion System in Pseudomonas aeruginosa Pseudomonas aeruginosa, an opportunistic pathogen, commonly colonizes patients with cystic fibrosis (1) and contributes to a sizeable proportion of antibiotic-resistant nosocomial infections (2). Acute P. aeruginosa infections are characterized by the expression of the type III secretion system (T3SS) comprised of a needle-like apparatus used to inject exoenzymes into neighboring cells (1, 3). These…

Different methods for purification of plasmid exist; during this laboratory experiment specifically kits were used (Qiagen kit). Kits provide the detailed procedures to follow step-by-step and are simple and quicker to perform. This system serves as a fast and simple plasmid purification method for routine molecular biology laboratory applications (Qiagen). This procedure is based on alkaline lysis method…

BSC1010L SectionU42 The effect of Control Plasmid and Plasmid Lux in Bacterial Transformation Vanessa Louis PID: 4709178 Lab Partners: Patrick Abaunza Raquel Alespeiti Robert Lugo Date Performed: 31 October 2014 TA: Reinier Llorca Abstract According to The General Biology I Lab manual, involved in Gene expression are two complex cellular pathways, each being subjected to regulation (Alberte et al., 2012). The first pathway is the synthesis of an RNA molecule from a DNA strand.…

This plasmid is a circular DNA molecule that is commonly used as a cloning vector with E.coli (Ke et al., 2008). This plasmid has been engineered to include a unique feature of a gene that codes for antibiotic resistance to ampicillin, an antibiotic that inhibits the synthesis of the cell wall which in result, destroys…

ampicillin environment then the plasmids triumphantly transformed the cell and as a result were able to create either the enzyme beta-lactamase, for the pUC18 transformed cells, or the enzyme luciferase, for the lux transformed cells, which would counteract the effect of ampicillin on the cell wall of the bacteria. There were many errors in this experiment. Only two of the agar plates actually developed properly, one of them was the ampicillin agar plate with no plasmid (Fig 1: top left) because…

Plasmids are extra chromosomal DNA within some bacteria that exist as circular structures. Although they are not essential for survival of the bacterium, they often do confer many environment advantages such as antibiotic resistance. One bacteria of interest is E. coli, a gram-negative organism that is part of normal human gut flora. Some strains of E. coli are pathologic to humans, however some strains are beneficial. For example, one strain produces vitamin K, an essential nutrient. In this…