Transformation

Genetic transformation is a process by which competent bacteria intakes naked DNA from the surrounding environment and incorporates the foreign DNA into its own genetic code. Transformation only occurs in competent bacteria. A naturally competent bacterium is classified as a bacterium that can readily intake naked DNA. Fortunately, the bacteria that are not naturally competent can still undergo transformation by being treated with various agents to artificially induce competence. Also, another factor that plays a role in transformation is plasmids. Plasmids are small circular pieces of DNA that assist with gene transfer in artificially induced transformation. In this lab, a previously made plasmid called pGLO was used to conduct the transformation process into Escherichia coli (E. coli). pGLO carries several genes such as β-lactamase, green fluorescent protein, origin of replication, and transcriptional regulator gene that affects transformation. For instance, the β-lactamase gene (bla) gives the transformed bacteria resistance to ampicillin. The green fluorescent protein gene (gfp) will provide the transformed bacteria with a green glow. The origin of…

How is it that people can look around at other individuals and watch them transform right in front of their eyes? In the movie Finding Forrester people are able to watch many characters transform just due to the human beings around them. Jamal has a strong passion for writing but, is always looking for help to improve. William is a writer who has shut himself away from society. These two happen to have a truly unique meeting, Jamal breaks into William's apartment due to a dare, from this point…

Transformation is the first step in accepting one’s true potential. In Finding Forrester, Jamal, a talented high schooler, meets William Forrester, a hermit author. At first they seem to be two sides of the same coin, but as conflict occurs they rise above their fears and grow for the better. In chaotic confrontations, people’s faults become apparent, and they transform into who they really are. During confrontation, people realize their transformation into who they really are. Professor…

organization. This is a critical first step as it determines how willing a client is willing to accept change which is normally an indicator for a successful candidate in a business partner relationship. Moving from a traditional fee-for-service model which provides a predictable revenue for a health system to a value-based payment system requires a radical change not only in how the health system operates but also in their willingness to undergo a transformation…

ride my friends told me to go on. When i was in the line i got so scared that i ran out the line and i got a stomach ache and i called my brother to pick me up. It started as a fun and interesting day with my friends and the it transformed to a horrible day which i will never forget. Oate’s “Where is Here” “Dream Collector” and Julio Cortaztar”s “House Taken Over” uses transformation of an ordinary person and an ordinary setting to show us how easily circumstances can change.…

One company began its transformation process with much publicity and actually made good progress through the fourth phase. Then the change effort ground to a halt because the officer in charge of the company’s largest division was allowed to undermine most of the new initiatives. He paid lip service to the process but did not change his behavior or encourage his managers to change. He did not reward the unconventional ideas called for in the vision. He allowed human resource systems to remain…

In the small desert town of Esperarcita, Mexico, the lives of its citizens mimic its name: those of a little hope, those of little hope, and those who continue to wait a little. This novel focuses on community, in particular the relationships among a small group of individuals and their specific challenges of transformation. An old cement park bench, with the inscription “ALBERT JOHNSON,” is a central character, that while serving as a meeting place of friends, through a subtle touch “he”…

a competent bacteria cell able to take up a pGLO plasmid by means of heat shock genetic transformation. To test this, colonies of E. coli mixed either with a pGLO plasmid or without a pGLO plasmid were placed on four Luria-Bertani (LB) agar nutrient plates. One plate had only LB, two had both LB and ampicillin, and one had LB, ampicillin and arabinose sugar. We hypothesized for this study that the E. coli mixed with the pGLO plasmid would take up the plasmid and would then thrive on the agar…

As the Editor’s note by Harvard Business Review pointed out on its review of Leading Change Why Transformation Efforts Fail by John Kotter, there are general lesson to be learned from the more successful cases of corporate change efforts. First is that the change process goes through a series of phases that, in total, usually require a considerable length of time. Skipping steps creates only the illusion of speed and never produces a satisfying result. Second, is that critical mistakes in any of…

In the poems “Medusa” by Duffy and “The Laboratory” by Browning both authors explore the theme of jealousy and its destructive nature on people and society as a whole. In Duffy’s poem “Medusa” she critiques society on its treatment towards women, demonstrating how those without beauty are only corrupted with jealousy and how this behavior has survived through the ages. While Duffy focuses on the impacts of jealousy on the individual Browning looks towards its impacts on society, and its power to…