Plasmid

BIOL 1010 ASSIGNMENT 1 OCT 6 BY JORDAN KAPITANY ST 100883963 Among the many scientific achievements of the twentieth century in the field of bio-technology scientists Paul Berg, Herbert W Boyer, Stanley N Cohen and team for their research that lead the party to discover a technique of taking genes from one organism and inserting them into another organism, also more formally known as Deoxyribonucleic Acid or DNA recombinant technology. In 1971 Berg and team successfully isolated DNA of…

outspreading from the surface of a cell. The cells that are the donor during conjugation have an F Plasmid (fertility plasmid), which is a circular, small, extrachromosomal molecule of DNA. Donor with cells are referred as F+ and recipient as F-. This process involves the following steps: 1) The pilus connects the donor cell (F+) and the recipient cell (F-). 2) Only a single strand of the F plasmid DNA is transfer to the recipient. 3) The recipient (F-)…

chosen restriction enzyme BsrG1. Tube A and Tube B were the negative controls, Tube A received 10ul of sterile water and 10ul of WT plasmid DNA. Tube B received 10ul or sterile water and 10ul of m2 Mutant plasmid DNA. Tube C and Tube D were the positive controls. Tube C and D both received 4ul of sterile water and reaction buffer. Tube C was also given 10ul of WT plasmid DNA and 2ul of the…

sites from one another. After cutting up the dsDNA into smaller fragments, they usually undergo electrophoresis so data can be obtained. Small quantities of nucleic acid can be detected through agarose gel electrophoresis. Samples with the desired plasmid, DNA loading dye, restriction enzymes, and water are mixed and carefully pipetted into wells on an agarose gel. There will also be a well with a DNA marker to help determine the relative sizes of the fragments. A…

discussing the experiment, Cohen realized that, “ Eco RI was the missing ingredient needed for the molecular analysis of antibiotic resistance plasmids.”(DNA Cloning) He states that, large plasmids could be cut specifically and reproducibly by the enzyme, and that this method could be better than what he has been using the past few years for fragmentation of plasmid DNA…

NTPS which will preserve our DNA specimen. To put the gene into the plasmid PGEM3Z, we will digest the DNA by using restriction enzymes such as Xba I to cut the DNA into smaller fragments containing the EPAS1-TD gene. The restriction enzymes will also cut at the designated restriction enzyme cleavage site in the plasmid where it will transfer the cut up EPAS1 gene into the plasmid PGEM3Z. The DNA Ligase will come and seal the plasmid shut with the EPAS1 gene DNA segment in it. Doing this will…

specific points at the restriction site. Lastly, the DNA ligase joins the DNA from two different sources and produces a recombinant DNA molecule by catalyzing the formation of covalent bonds that close up the sugar-phosphate backbones. Bacterial plasmids are used to clone DNA sequences of interest and then create bacterial strains which produce a human protein in large amounts by having…

viruses with part of the genome inactivated or knocked out. They can also be used as vectors or as infectious clones of a disease agent. Recombinant inactivated vaccines only contain part of a whole organism while genetic vaccines are recombinant plasmids made from purified bacteria. These recombinant vaccines are used for humans, swine, poultry, and sheep. Point mutations can also be induced by the size, location, and type of insertion or deletion in recombinant DNA. Recent developments have…

A DNA ladder is a solution of DNA molecules of different but known length. In Well 1, the DNA ladder was applied to the gel to set a standard for the rest of the samples. In LoggerPro, after setting an origin, the second step was to set a standard ladder. We standardized the ladder by going from 4 to 5 cm and determining that was 10 milliliters. The next step was to scale the ladder by selecting the 3 brightest points and labeling it 3000,1000,500 in descending order. The estimated size of my…

antibiotics, but not the plasmid had no growth because the bacteria was not introduced to the plasmid that had the antibiotic resistant gene.The LB/amp had growth to it because it was not introduced to any new molecules or chemicals. The po The other plates had growth because they was introduced to the antibiotic resistant gene that made the bacteria able to grow. The positive control of the experiment was the LB plate, because the environment was not changed by any plasmids or antibiotics,…