Genetic transformation is an important method, in molecular biology and genetic engineering, for transferring DNA amongst a variety of organisms. In Lab five, my lab partners and I used calcium chloride to make the bacterium cell walls more permeable and a heat shocking method to introduce the pGLO plasmid in the E.coli bacterium so that they may exhibit ampicillin resistance. The Goal of the experiment was to observe whether or not, given one of the four specific conditions, the pGLO plasmid would be able to transcribe itself into the E.coli bacterium. If the cells were successful in up-taking the plasmid, then they would inherently become resistant to ampicillin, be able to grow into colonies, and exhibit the Green Florescence Protein under
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Instead of partitioning into separate colonies, like the bacteria in figure one, the bacterial in figure three have dispersed throughout the entire petri dish and created an indistinguishable flat lawn of bacteria.
Figure 4, Figure # 4: LB/amp: + PGLO, exhibited growth much like the bacteria in figure one. The only difference I noticed was that there were about two third the amount of colonies, with respect to figure one, each colony was much smaller when compared side by side did not glow green in the presence of ultra violet light.
Discussion/ conclusion:
Our objective for this experiment was to genetically modify E.coli bacterium so that they will be able to transcribe pGLO plasmids in an attempt to build up ampicillin resistance and to be able to observe the colonies glow bright green. In proper conditions, bacteria that have successfully transcribed the +pGLO plasmids are resistant to the ampicillin within the agar gel. If the transformed E.coli bacterium are in the presence of arabinose, they form colonies that are able to glow green when held under UV light. The bacterium in the
Instead of partitioning into separate colonies, like the bacteria in figure one, the bacterial in figure three have dispersed throughout the entire petri dish and created an indistinguishable flat lawn of bacteria.
Figure 4, Figure # 4: LB/amp: + PGLO, exhibited growth much like the bacteria in figure one. The only difference I noticed was that there were about two third the amount of colonies, with respect to figure one, each colony was much smaller when compared side by side did not glow green in the presence of ultra violet light.
Discussion/ conclusion:
Our objective for this experiment was to genetically modify E.coli bacterium so that they will be able to transcribe pGLO plasmids in an attempt to build up ampicillin resistance and to be able to observe the colonies glow bright green. In proper conditions, bacteria that have successfully transcribed the +pGLO plasmids are resistant to the ampicillin within the agar gel. If the transformed E.coli bacterium are in the presence of arabinose, they form colonies that are able to glow green when held under UV light. The bacterium in the