Plasmid

Research Question: How does the mass (g) of soap in the Extraction solution affect the mass of DNA extracted (g) from kiwi? Aim: To see how the mass of soap in the extraction solution affect the mass of DNA extracted from a kiwi. Background Research: DNA is found in the nucleus of the cell. For this experiment, the main goals is to break apart the plant matter, dissolve the membranes of the cell and extracting the DNA found in the nucleus . To extract DNA, we would need a kiwi as DNA exists…

Molecular communication is a paradigm for Nanomachines to exchange information. Due to some of the biological properties, bacteria have been proposed as a carrier for molecular communication, such communication networks are known as bacterial Nanonetworks. The biological property of bacteria is ability to mobilize and carry the information encoded in deoxyribonucleic acid molecules. Bacteria have social characteristic, which provides bacteria to evolve in fluctuating environment using…

E. coli Genomic DNA Isolation and Quantitation Introduction In this experiment, the genomic DNA of E. coli cells were isolated, purified, and quantified. To achieve this, E. coli cells were lysed, purified from impurities and Methods E. coli cells were first centrifuged in order to separate the cell pellet from the supernatant. Cell pellets were resuspended in saline-EDTA, which weakens bacterial membranes and chelates metal ions. Since E. coli has three layers (outer membrane,…

3.4 Case Studies 3.4.1 Biofuel from GM Macro algae Hydrocarbon biofuels are produced by genetically modified seaweed which are obtained by inserting genes from high hydrocarbon producing micro algae into high growth seaweed species. Botryococcus braunii (BB) a green micro algae that produces large amounts of aliphatic hydrocarbon molecules is used as a source for genetic material. The genes that produce hydrocarbon in BB are identified, removed, cloned and subsequently inserted into high…

DNA analysis, also known as DNA profiling, testing, typing, is a process that takes genetic material and evaluates it so that it can identify individuals in a criminal investigation or in use of a forensic application. The beginning step of the performance of DNA analysis on a reference sample or person is the collection of DNA from cells. These cells can come from a blood sample or even swabbing the inside of an individual’s cheek. After it is collected, the samples are then sent to a lab for…

Assignment #2: The Replication of DNA in Escherichia Coli Due Date: Feb 8, 2016 at 9:00 AM CST 1a. A procedure in which a small amount of DNA material is immersed in concentrated solution, cesium chloride (CsCl), and soon after placed in centrifuge until the point where equilibrium is achieved is called density-gradient centrifugation. Contrasting techniques involving diffusion and sedimentation form a persisting CsCl concentration gradient. Furthermore, the concentration gradient and pressure…

In this experiment, the hypothesis gave the expected results. The positive DNA has pGLO that can grow regardless that has the presence of the Ampicillin. The negative DNA does not have pGLO and thus cannot grow in the presence of Ampicillin because pGLO contains ampicillin-resistance gene. When it is not pGLO breaks the Ampicillin. The LB does not have Ampicillin and can grow colonies on the agar plates. For example, in the LB (- DNA) shown large number of white because contain negative DNA but…

All cases of the E.coli outbreak diagnosed among residents of Michigan from June 15th to July 15th 1997 were identified by the michigan Department of Community Health based on laboratory reports. The first step we took to prove our hypothesis was to consider why there was an increase in reported cases and if the possible outbreak was due to contaminated food, water contamination or person to person contact. The information we needed to determine which of these explanations was likely the cause…

The effects of the catecholamines epinephrine and norepinephrine on different anaerobic bacteria are given in Figure 1. 3.2.1. Change in bacterial gene expression Important effect of the catecholamines is the change in bacterial gene expression. Saito et al. [29] observed the effect of catecholamines on P. gingivalis virulence factor expression by a microarray and reverse transcription polymerase chain reaction (RT-PCR). The addition of norepinephrine resulted in a decrease in P. gingivalis…

Have you heard all the talk about peptides and their incredible anti-aging effect? Peptide anti-aging serums are the biggest sensation to hit the anti-aging skincare market in years. So what is a peptide, and how does it work? Peptides are the naturally occurring building blocks of the skin. A peptide is a small protein fragment that is joined by two or more amino acids. They can be derived naturally or lab simulated. Peptides act as the messengers between skin cells carrying important messages…