Abstract Many number of medicines, like growth hormones and insulin, are a possibility because of techniques like transformation of genetically engineered bacteria. Although, manipulating bacterial gene expression has many great implications, it can also be quite harmful. By manipulating gene expression of bacteria, such as Escherichia coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was conducted in order to transform E.coli into an antibiotic-resistant bacteria by introducing a plasmid, lux and pUC18, containing a gene for the resistance to Ampicillin. The procedure had four basic steps: treating bacterial cells with CaCl2 solution in
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One industry that is certainly reaping its benefits is the healthcare industry, which has biotechnology to be able make insulin, antibiotics, vaccines and growth hormones (Alberte et al., 2012). Another industry benefiting from biotechnology is the agriculture industry, which has utilized biotechnology to produce crops resistant to disease and has allowed environmental preservation to be able to make products that are biodegradable (Alberte et al., 2012). Biotechnology also has great implications for the future, including the development of whole cell biosensors with the capacity to screen and analyze pharmaceuticals and food products for the detection of microbial contamination (Shah, …show more content…
DNA is normally referred to as nuclear DNA, but can be found in other forms, such as plasmids (Alberte et al., 2012). Plasmids are small molecules with a circular shape (Gohlmann & Talloen, 2010). Plasmids are not necessarily vital to for survival of host bacteria but do allow the bacteria to reproduce and survive in certain environments (Alberte et al., 2012). According to Gupta (2009), some plasmids are also known to assist bacteria in becoming resistant to antibiotics given. This is due to the fact that they contain certain genes, that when expressed, make them resistant (Alberte et al., 2012). If a bacterial cell contains this kind of plasmid, it then has the potential to survive and reproduce even if exposed to antibiotics (Locker,
One industry that is certainly reaping its benefits is the healthcare industry, which has biotechnology to be able make insulin, antibiotics, vaccines and growth hormones (Alberte et al., 2012). Another industry benefiting from biotechnology is the agriculture industry, which has utilized biotechnology to produce crops resistant to disease and has allowed environmental preservation to be able to make products that are biodegradable (Alberte et al., 2012). Biotechnology also has great implications for the future, including the development of whole cell biosensors with the capacity to screen and analyze pharmaceuticals and food products for the detection of microbial contamination (Shah, …show more content…
DNA is normally referred to as nuclear DNA, but can be found in other forms, such as plasmids (Alberte et al., 2012). Plasmids are small molecules with a circular shape (Gohlmann & Talloen, 2010). Plasmids are not necessarily vital to for survival of host bacteria but do allow the bacteria to reproduce and survive in certain environments (Alberte et al., 2012). According to Gupta (2009), some plasmids are also known to assist bacteria in becoming resistant to antibiotics given. This is due to the fact that they contain certain genes, that when expressed, make them resistant (Alberte et al., 2012). If a bacterial cell contains this kind of plasmid, it then has the potential to survive and reproduce even if exposed to antibiotics (Locker,