Escherichia coli

Purpose: To study the expression of the Vibrio fischeri luciferase operon in Escherichia coli (E. coli) by isolating and purifying a plasmid from E. coli, determining its orientation with respect to the plasmid backbone by restriction mapping, and transforming it into an alternate E. coli strain. Methods: Each individual received either E. coli A or B strain of pKN800 plasmid DNA. The plasmid was purified and isolated, then cut with PstI restriction enzyme to create a sample of PstI-cut and…

used to conduct the transformation process into Escherichia coli (E. coli). pGLO carries several genes such as β-lactamase, green fluorescent protein, origin of replication, and transcriptional regulator gene that affects transformation. For instance, the β-lactamase gene (bla) gives the transformed bacteria resistance to ampicillin. The green fluorescent protein gene (gfp) will provide the transformed bacteria with a green glow. The origin of…

Klebsiella pneumoniae, Salmonellae, shigallae, Haemophilus influenza, Pasteuerella, Neisseria meningitides, Vibro cholera, Bordetella pertussis, Bacillus anthracis, Corynebacterium diptheriae, Streptococcus faecalis, Listeria monocytogenes, Escherichia coli Mycobacterium tuberculosis, Borrelia and Leptospira interrogans (L. icterohemorrhagicae). Despite having the combination of bacitracin with neomycin provide a broad-spectrum effect, they do not cover Nocardia, Pseudomonas, fungi and viruses.…

Throughout the world, millions of crops are being affected by Escherichia Coli Bacteria, even though some strains of E. coli are harmless and cause mild illnesses other strains can be extremely dangerous to humans. The Centers for Disease Control (CDC) states there about two major outbreaks every year which can infect an estimate of 265,000 people. This bacteria, which can outbreak anywhere around the world due to the various sources of the infection, can stay dormant in humans for months…

Escherichia coli (E. coli) is one of the most widely studied and understood living organisms. It has been used for decades in furthering understanding of gene functionality and gene-protein mechanisms for prokaryotes and eukaryotes alike (Riley et al. 2006). Prokaryotes, like E. coli, have their DNA in the form of a plasmid. A plasmid is a circular ring of DNA. The plasmid intended to transform the E. coli colonies for the purposes of this study was the pGLO plasmid. The pGLO plasmid contains…

2.0 INTRODUCTION Consumption of foods and beverages that do not have added preservative but able to have a longer shelf life has become very popular among the consumer. In order to improve the shelf life of a food product, adding natural food extracts that act as the antimicrobial are able to inhibit or supress the microbial growth (Martin et al 2009). Microbial growth, pH and the storage temperature are the important measure of the shelf life and quality of a food product (Fellers 1988;…

UTIs primarily effect women. To begin, a Urinary Tract Infection is an infection of the urethra, bladder, or kidneys and can be caused by several different types of bacteria, by far the most common being Escherichia coli, or in other terms E. coli (Djordjevic). More times than not, the E. coli bacteria is able to get to the urethra from the anus through poor hygiene (Wexler), spreading through sexual activity, and can even be transmitted to the urethra by wearing thongs. First off, hygiene is…

(1997) also isolated ferritin via protein expression of pET3 vector that contains 5F12 coding sequence in the NdeI sequence site for the H-type subunit of the frog ferritin. The vector host was Escherichia coli BL21-3 cells grown in medium containing sorbital and betaine. Ferritin synthesis was induced in mid-log cells using isothiopropyl thiogalactoside (IPTG), and the soluble ferritin was isolated, after sonication of the bacterial cells, by fast…

Introduction Escherichia coli or more commonly known as E. Coli is a gram negative, facultative anaerobic bacterium that is commonly found in the lower intestine. There are many different strains of E. Coli, with strains that are created in a laboratory setting that are used as a standard for testing other strains of e.coli.1 These special strains possess an inherent tetracycline resistance from the insertion of a “marker” 2’ upstream or downstream from a targeted gene. These tetracycline marked…

male patient admitted on 2/22/2016 for infected stage IV decubitus ulcers, and penile deformity caused by urethral erosion from indwelling catheter. Patient is also anemic, and is on isolation precaution due to MRSA, staphylococcus aureus, and Escherichia coli infections. M.P. is a homeless paraplegic with double above the knee amputations (due to a motor vehicle accident in 2013) with an opiate addiction suffering from severe malnutrition, extreme depression, and failure to thrive. He has…