Which household cleaning product (hydrogen peroxide, Pine-sol, Clorox Bleach, Mr Clean antibacterial liquid cleaner) is the best inhibitor of K12 Escherichia coli (E. coli) growth? The manipulated variable was the cleaning product or disinfectant on used of the Whatman antibiotic assay paper disc. The dependent variable is the zone of inhibition of the prokaryotic bacteria E. coli. In topic one IB requires that we learn about Eubacteria, traditional bacteria that are typically pathogenic, E. coli fall into this category. We also had to learn about Florey and Chain experiments, which is where I learned about zones of inhibition. The problem interests me because is important that one knows which cleaning product to use in one household to get rid of the most bacteria. The idea is that if one test the most affective cleaning product on bacteria that is not harmful one can determine the most effective cleaning product to use in ones home it so that the most harmful bacteria can be killed. …show more content…
Coli is a commonly found bacteria in the environment, food, and the intestines of human and animals. Most strains are harmless, but some strain can make humans sick, food poisoning, diarrhea, urinary tract infections and respiratory illnesses.
E. coli K12 strain is not known for any harmful effects. The K12 is considered preferable because it has a more rapid and consistent growth. IB requires that you cannot grow bacteria at Room temperature. E. coli grow best at 37 degrees Celsius so that is the temperature I will grow it at in the incubator.
Hydrogen peroxide, H2O2, is sold to consumers in 3% to 6% concentration in water. When bacteria cause the immune system to activate specific cells produce large amounts of Hydrogen peroxide to fight the infection. It is a disinfectant and an