Agar plate

positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test using a peptone-iron agar deep. The results of the H2S production test showed no production of H2S near the inoculation point…

2.0 Introduction Consumption of foods and beverages that encourages a healthy living, better health and lowering the risk of sickness has become very popular among the consumer whom are health conscious. Hence, a lot more attention is paid to different food extracts such as green tea extracts, grape seed extracts and ginger extracts as they posed excellent antimicrobial, antioxidant and anti-carcinogenic properties (Martin et al. 2009). These extracts have great potential in improving the…

Introduction The purpose of this unknown lab is to separate and identify two unknown microbes from a mixed culture. Kathryn Bart define a mixed culture as "one that contains more than one type of organism growing in a sterile medium, such as agar. The mixed culture can include multiple species of viruses, bacteria and parasites, which may or may not live in harmony with one another, sharing the available resources" (Kathryn Barr, 2016). I took number five test tube from the different microbes…

(3). This lab uses different types of media that are both selective and differential. Mannitol Salt agar (MSA), which contains high levels of sodium chloride kills most bacteria that are not members of the halotolerant Staphylococcus genus. MSA also contains mannitol, which allows differential identification because an indicator detects acid produced by the fermentation of mannitol (4). MacConkey agar, which is media containing crystal violet and bile salts inhibits Gram positive bacteria (5).…

Identification of Human Streptococcal Pathogens Materials 1. Glass microscope slides 2. Bunsen burner 3. Inoculating loop 4. Sharpie pen 5. 2 bacitracin discs 6. Sterile saline tubes 7. Sterile cotton swabs 8. Tongue depressors 9. Gloves 10. 1 blood agar plate/student pair Procedure Divide a blood agr into two sections, one for you and the other for the lab partner. Wash hand thoroughly before proceduring to the getting a throat culture. Depress the tongue with a sterile depressor, donot put it…

broth, a deep, an agar slant and a streak plate are all inoculated with the unknown bacterium via a needle for the deep and a loop for the broth, agar slant and streak plate. After forty-eight hours all the mediums are examined to see what growth has occurred and the characteristics of growth (if any) are documented. This bacterium was found to be motile from results via the deep, a thin film was visible atop the broth, and sediment was seen at the bottom, with some growth on the agar slant. The…

2.0 INTRODUCTION Consumption of foods and beverages that do not have added preservative but able to have a longer shelf life has become very popular among the consumer. In order to improve the shelf life of a food product, adding natural food extracts that act as the antimicrobial are able to inhibit or supress the microbial growth (Martin et al 2009). Microbial growth, pH and the storage temperature are the important measure of the shelf life and quality of a food product (Fellers 1988;…

chose to streak a Sheep’s Blood Agar plate first. My thinking was if I were to observe weak beta hemolysis then I would have either Staphylococcus aureas or Streptococcus pyogenes. If the results were gamma then I would eliminate Staphylococcus aureas and Streptococcus pyogenes and narrow down my organism to Staphylococcus epidermidis, Enterococcus faecalis, or Sarcina lutea. After two days I returned to the lab to discover my organism exhibits gamma…

Impact of penicillin, novobiocin, and kanamycin on Staphylococcus aureus bacterial growth Introduction: In today’s world, bacteria are becoming more abundant and strong in terms of their resistance to antibiotics. As a result of increasing prescription potency, clinicians are supposed to be conscious of those patients who are more inclined to infection by prescribing the appropriate antibiotics on the basis of their sensitivities along with their cultures (Fleming et al., 2006). A recent case…

Dettol. This is show in the data displayed above in both the Table and Graph. From the data collected it was discovered that tea tree oil wasn’t the best chemical to use to inhibit the growth of the bacteria. It was found that on all three of the agar plates almost all of the surface area around the paper disk was inhibited. The chemical that appeared to be the most effective at inhibiting the growth of bacteria was the Dettol. On all the samples from this chemical, no bacteria grew all…